3QSK

5 Histidine Variant of the anti-RNase A VHH in Complex with RNAse A

3QSK の概要

• エントリーDOI: 10.2210/pdb3qsk/pdb
• 関連するPDBエントリー: 1BZQ 2P49
• 分子名称: Ribonuclease pancreatic, Engineered 5 Histidine anti-RNase A Camelid VHH Antibody Domain Variant (3 entities in total)
• 機能のキーワード: antibody, single domain, camelid, vhh, sdab, rnase a, ribonuclease a, ph, sensitivity, switch, dependence, linked, equilibria, equilibrium, protein engineering, combinatorial, histidine, scanning, phage display, dual-function, protein-protein interaction, proton binding, hydrolase-immune system complex, hydrolase/immune system
• 由来する生物種: Camelus dromedarius (Arabian camel); 詳細
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26815.80

構造登録者

Murtaugh, M.L.,Fanning, S.W.,Sharma, T.M.,Terry, A.M.,Horn, J.R. (登録日: 2011-02-21, 公開日: 2011-08-10, 最終更新日: 2011-11-30)

• 主引用文献: Murtaugh, M.L.,Fanning, S.W.,Sharma, T.M.,Terry, A.M.,Horn, J.R.; A combinatorial histidine scanning library approach to engineer highly pH-dependent protein switches.; Protein Sci., 20:1619-1631, 2011; PubMed: 21766385; DOI: 10.1002/pro.696

実験手法

X-RAY DIFFRACTION (1.75 Å)