3QO6

Crystal structure analysis of the plant protease Deg1

Summary for 3QO6

Entry DOI	10.2210/pdb3qo6/pdb
Descriptor	Protease Do-like 1, chloroplastic, peptide, ... (6 entities in total)
Functional Keywords	protease, htra, ph-sensor, hydrolase, photosynthesis
Biological source	Arabidopsis thaliana (Thale-cress) More
Cellular location	Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: O22609
Total number of polymer chains	9
Total formula weight	112762.44
Authors	Clausen, T. (deposition date: 2011-02-09, release date: 2011-05-04, Last modification date: 2024-11-06)
Primary citation	Kley, J.,Schmidt, B.,Boyanov, B.,Stolt-Bergner, P.C.,Kirk, R.,Ehrmann, M.,Knopf, R.R.,Naveh, L.,Adam, Z.,Clausen, T. Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure. Nat.Struct.Mol.Biol., 18:728-731, 2011 Cited by PubMed Abstract: Deg1 is a chloroplastic protease involved in maintaining the photosynthetic machinery. Structural and biochemical analyses reveal that the inactive Deg1 monomer is transformed into the proteolytically active hexamer at acidic pH. The change in pH is sensed by His244, which upon protonation, repositions a specific helix to trigger oligomerization. This system ensures selective activation of Deg1 during daylight, when acidification of the thylakoid lumen occurs and photosynthetic proteins are damaged. PubMed: 21532594 DOI: 10.1038/nsmb.2055 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.5 Å)

Structure validation