3QKZ

Crystal structure of mutant His269Arg AKR1B14

3QKZ の概要

• エントリーDOI: 10.2210/pdb3qkz/pdb
• 関連するPDBエントリー: 3O3R
• 分子名称: Aldo-keto reductase family 1, member B7, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total)
• 機能のキーワード: aldose reductase-like proteins, akr1b14, oxidoreductase
• 由来する生物種: Rattus norvegicus (rat)
• 細胞内の位置: Cytoplasm (By similarity): Q5RJP0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73872.43

構造登録者

Sundaram, K.,El-Kabbani, O. (登録日: 2011-02-02, 公開日: 2012-02-29, 最終更新日: 2023-11-01)

主引用文献

Sundaram, K.,Endo, S.,Matsunaga, T.,Tanaka, N.,Hara, A.,El-Kabbani, O.
Structure of the His269Arg mutant of the rat aldose reductase-like protein AKR1B14 complexed with NADPH.
Acta Crystallogr.,Sect.F, 68:400-403, 2012

PubMed Abstract: Rat aldose reductase-like protein (AKR1B14) is an orthologue of mouse vas deferens protein (AKR1B7) and plays roles in the detoxification of reactive aldehydes and synthesis of prostaglandin F(2α). Here, the 1.87 Å resolution crystal structure of the His269Arg mutant of AKR1B14 complexed with NADPH is described and shows that the negatively charged 2'-phosphate group of the coenzyme forms an ionic interaction with the positively charged guanidinium group of Arg269 that is also observed in the human aldose reductase (AKR1B1) structure. Previous experiments on the site-directed mutagenesis of His269 to Arg, Phe and Met revealed fourfold, sevenfold and 127-fold increases in the K(m) for NADPH, respectively, which are in agreement with the present molecular-modelling and X-ray crystallographic studies. This is the first tertiary structure of a mutant form of this AKR1B7 orthologue to be reported in order to investigate the structure-function relationship of the nonconserved His269 and its role in coenzyme binding.

PubMed: 22505406
DOI: 10.1107/S1744309112008810

実験手法

X-RAY DIFFRACTION (1.87 Å)