3QA9

Crystal Structure of Prb (PH1109 protein redesigned for binding)

3QA9 の概要

• エントリーDOI: 10.2210/pdb3qa9/pdb
• 関連するPDBエントリー: 3Q9N 3Q9U
• 分子名称: CoA binding domain protein (2 entities in total)
• 機能のキーワード: prb monomer, coa binding motif, engineered binding partner for pdar, pdar, protein binding, de novo protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17398.13

構造登録者

Spiegel, P.C. (登録日: 2011-01-10, 公開日: 2011-04-20, 最終更新日: 2024-02-21)

主引用文献

Karanicolas, J.,Corn, J.E.,Chen, I.,Joachimiak, L.A.,Dym, O.,Peck, S.H.,Albeck, S.,Unger, T.,Hu, W.,Liu, G.,Delbecq, S.,Montelione, G.T.,Spiegel, C.P.,Liu, D.R.,Baker, D.
A de novo protein binding pair by computational design and directed evolution.
Mol.Cell, 42:250-260, 2011

PubMed Abstract: The de novo design of protein-protein interfaces is a stringent test of our understanding of the principles underlying protein-protein interactions and would enable unique approaches to biological and medical challenges. Here we describe a motif-based method to computationally design protein-protein complexes with native-like interface composition and interaction density. Using this method we designed a pair of proteins, Prb and Pdar, that heterodimerize with a Kd of 130 nM, 1000-fold tighter than any previously designed de novo protein-protein complex. Directed evolution identified two point mutations that improve affinity to 180 pM. Crystal structures of an affinity-matured complex reveal binding is entirely through the designed interface residues. Surprisingly, in the in vitro evolved complex one of the partners is rotated 180° relative to the original design model, yet still maintains the central computationally designed hotspot interaction and preserves the character of many peripheral interactions. This work demonstrates that high-affinity protein interfaces can be created by designing complementary interaction surfaces on two noninteracting partners and underscores remaining challenges.

PubMed: 21458342
DOI: 10.1016/j.molcel.2011.03.010

実験手法

X-RAY DIFFRACTION (1.9 Å)