3QA9
Crystal Structure of Prb (PH1109 protein redesigned for binding)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.003, 59.395, 60.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 1.900 |
| Rwork | 0.213 |
| R-free | 0.24170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.124 | |
| Number of reflections | 11519 | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 97.9 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 10 mg/mL, 0.1 M Na-acetate, 3.0 M NaCl, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
