3QA9
Crystal Structure of Prb (PH1109 protein redesigned for binding)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.003, 59.395, 60.038 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.000 - 1.900 |
Rwork | 0.213 |
R-free | 0.24170 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.124 | |
Number of reflections | 11519 | |
<I/σ(I)> | 8.1 | |
Completeness [%] | 97.9 | 96.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 10 mg/mL, 0.1 M Na-acetate, 3.0 M NaCl, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |