3Q8L

Crystal Structure of Human Flap Endonuclease FEN1 (WT) in complex with substrate 5'-flap DNA, SM3+, and K+

Summary for 3Q8L

• Entry DOI: 10.2210/pdb3q8l/pdb
• Related: 3Q8K 3Q8M
• Descriptor: Flap endonuclease 1, DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3'), DNA (5'-D(*TP*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3'), ... (8 entities in total)
• Functional Keywords: helix-3 turn-helix, hydrophobic wedge, 3' flap binding site, hydrolase-dna complex, dna repair, replication, flap endonuclease, fen, fen1, dna, nuclease, 5' flap, ss-dsdna junction, helix-2 turn-helix, h2th, h3th, divalent cation, metal helical gateway, cap, acid block, two metal mechanism, unpaired, 5' nuclease, human, long patch base excision repair, hydrolase/dna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus, nucleolus: P39748
• Total number of polymer chains: 4
• Total formula weight: 50621.23

Authors

Tsutakawa, S.E.,Classen, S.,Chapados, B.R.,Arvai, A.,Finger, D.L.,Guenther, G.,Tomlinson, C.G.,Thompson, P.,Sarker, A.H.,Shen, B.,Cooper, P.K.,Grasby, J.A.,Tainer, J.A. (deposition date: 2011-01-06, release date: 2011-04-27, Last modification date: 2023-09-13)

• Primary citation: Tsutakawa, S.E.,Classen, S.,Chapados, B.R.,Arvai, A.S.,Finger, L.D.,Guenther, G.,Tomlinson, C.G.,Thompson, P.,Sarker, A.H.,Shen, B.,Cooper, P.K.,Grasby, J.A.,Tainer, J.A.; Human Flap Endonuclease Structures, DNA Double-Base Flipping, and a Unified Understanding of the FEN1 Superfamily.; Cell(Cambridge,Mass.), 145:198-211, 2011; PubMed: 21496641; DOI: 10.1016/j.cell.2011.03.004

Experimental method

X-RAY DIFFRACTION (2.319 Å)