3Q8L

Crystal Structure of Human Flap Endonuclease FEN1 (WT) in complex with substrate 5'-flap DNA, SM3+, and K+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Flap endonuclease 1	polymer	341	38511.2	1	UniProt (P39748) Pfam (PF00752) Pfam (PF00867)	Homo sapiens (human)	FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1
2	B (D)	DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3')	polymer	18	5476.6	1
3	C (E)	DNA (5'-D(*TP*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3')	polymer	12	3742.5	1
4	D (F)	DNA (5'-D(*AP*CP*CP*GP*TP*CP*C)-3')	polymer	7	2058.4	1
5	E, F, G, H (A)	SAMARIUM (III) ION	non-polymer		150.4	4	Chemie (SM)
6	I (A)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
7	J, K (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
8	L, M, N, O (A, D, E, F)	water	water		18.0	402	Chemie (HOH)

Sequence modifications

A: 2 - 336 (UniProt: P39748)

PDB	External Database	Details
Leu 337	-	expression tag
Glu 338	-	expression tag
Val 339	-	expression tag
Leu 340	-	expression tag
Phe 341	-	expression tag
Gln 342	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	49788.6
Non-Polymers*	Number of molecules	7
	Total formula weight	832.7
All*	Total formula weight	50621.2

*Water molecules are not included.