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3Q7V

Beta-Lactam-Sensor Domain of BlaR1 (Apo) from Staphylococcus Aureus with Carboxylated Lys392

Summary for 3Q7V
Entry DOI10.2210/pdb3q7v/pdb
Related1XA1 3Q7Z 3Q81 3Q82
DescriptorBeta-lactamase regulatory protein BlaR1, SULFATE ION, GLYCEROL, ... (5 entities in total)
Functional Keywordsantibiotic-binding, mrsa, hydrolase regulator
Biological sourceStaphylococcus aureus
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Total number of polymer chains2
Total formula weight59966.14
Authors
Borbulevych, O.Y.,Mobashery, S.,Baker, B.M. (deposition date: 2011-01-05, release date: 2011-06-29, Last modification date: 2013-01-09)
Primary citationBorbulevych, O.,Kumarasiri, M.,Wilson, B.,Llarrull, L.I.,Lee, M.,Hesek, D.,Shi, Q.,Peng, J.,Baker, B.M.,Mobashery, S.
Lysine Nzeta-decarboxylation switch and activation of the beta-lactam sensor domain of BlaR1 protein of methicillin-resistant Staphylococcus aureus.
J.Biol.Chem., 286:31466-31472, 2011
Cited by
PubMed Abstract: The integral membrane protein BlaR1 of methicillin-resistant Staphylococcus aureus senses the presence of β-lactam antibiotics in the milieu and transduces the information to the cytoplasm, where the biochemical events that unleash induction of antibiotic resistance mechanisms take place. We report herein by two-dimensional and three-dimensional NMR experiments of the sensor domain of BlaR1 in solution and by determination of an x-ray structure for the apo protein that Lys-392 of the antibiotic-binding site is posttranslationally modified by N(ζ)-carboxylation. Additional crystallographic and NMR data reveal that on acylation of Ser-389 by antibiotics, Lys-392 experiences N(ζ)-decarboxylation. This unique process, termed the lysine N(ζ)-decarboxylation switch, arrests the sensor domain in the activated ("on") state, necessary for signal transduction and all the subsequent biochemical processes. We present structural information on how this receptor activation process takes place, imparting longevity to the antibiotic-receptor complex that is needed for the induction of the antibiotic-resistant phenotype in methicillin-resistant S. aureus.
PubMed: 21775440
DOI: 10.1074/jbc.M111.252189
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.1 Å)
Structure validation

226707

數據於2024-10-30公開中

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