3Q7K

Formate Channel FocA from Salmonella typhimurium

Summary for 3Q7K

• Entry DOI: 10.2210/pdb3q7k/pdb
• Descriptor: Probable formate transporter, FORMIC ACID (3 entities in total)
• Functional Keywords: membrane protein, transport, cytoplasmic membrane, transport protein
• Biological source: Salmonella enterica subsp. enterica serovar Typhi
• Total number of polymer chains: 10
• Total formula weight: 321216.38

Authors

Lue, W.,Du, J.,Wacker, T.,Gerbig-Smentek, E.,Andrade, S.L.A.,Einsle, O. (deposition date: 2011-01-05, release date: 2011-04-27, Last modification date: 2023-11-01)

Primary citation

Lu, W.,Du, J.,Wacker, T.,Gerbig-Smentek, E.,Andrade, S.L.,Einsle, O.
pH-dependent gating in a FocA formate channel
Science, 332:352-354, 2011

PubMed Abstract: The formate transporter FocA was described to switch its mode of operation from a passive export channel at high external pH to a secondary active formate/H(+) importer at low pH. The crystal structure of Salmonella typhimurium FocA at pH 4.0 shows that this switch involves a major rearrangement of the amino termini of individual protomers in the pentameric channel. The amino-terminal helices open or block transport in a concerted, cooperative action that indicates how FocA is gated in a pH-dependent way. Electrophysiological studies show that the protein acts as a specific formate channel at pH 7.0 and that it closes upon a shift of pH to 5.1.

PubMed: 21493860
DOI: 10.1126/science.1199098

Experimental method

X-RAY DIFFRACTION (2.8 Å)