3PQC

Crystal structure of Thermotoga maritima ribosome biogenesis GTP-binding protein EngB (YsxC/YihA) in complex with GDP

Summary for 3PQC

Entry DOI	10.2210/pdb3pqc/pdb
Related	3PR1
Descriptor	Probable GTP-binding protein engB, GUANOSINE-5'-DIPHOSPHATE, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
Functional Keywords	rossmann fold, gtpase, gtp binding, cell cycle, hydrolase
Biological source	Thermotoga maritima
Total number of polymer chains	2
Total formula weight	46073.48
Authors	Chan, K.H.,Wong, K.B. (deposition date: 2010-11-26, release date: 2011-06-08, Last modification date: 2023-11-01)
Primary citation	Chan, K.H.,Wong, K.B. Structure of an essential GTPase, YsxC, from Thermotoga maritima Acta Crystallogr.,Sect.F, 67:640-646, 2011 Cited by PubMed Abstract: YsxC belongs to the YihA family of TRAFAC class GTPases. The protein is involved in the biogenesis of ribosomes and is essential for the survival of a wide range of bacteria. Here, crystal structures of YsxC from Thermotoga maritima and its complex with GDP were determined at maximal resolutions of 2.3 and 1.9 Å, respectively. Major structural differences are observed in the switch I region, which is disordered in the apo form but exists in both an `open' and a `closed' conformation in the GDP-bound state. A comparison with the structure of the GMPPNP-YsxC complex from Bacillus subtilis provides insights into the mechanism of conformational change in the switch I and II regions upon hydrolysis of GTP. PubMed: 21636901 DOI: 10.1107/S1744309111011651 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.9 Å)

Structure validation