3PN1

Novel Bacterial NAD+-dependent DNA Ligase Inhibitors with Broad Spectrum Potency and Antibacterial Efficacy In Vivo

3PN1 の概要

• エントリーDOI: 10.2210/pdb3pn1/pdb
• 分子名称: DNA ligase, 2-(butylsulfanyl)adenosine, 1-(2,4-dimethylbenzyl)-6-oxo-1,6-dihydropyridine-3-carboxamide, ... (4 entities in total)
• 機能のキーワード: atp grasp fold, ligase, dna, bacteria, ligase-ligase inhibitor complex, ligase/ligase inhibitor
• 由来する生物種: Haemophilus influenzae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36411.29

構造登録者

Mills, S.,Eakin, A.,Buurman, E.,Newman, J.,Gao, N.,Huynh, H.,Johnson, K.,Lahiri, S.,Shapiro, A.,Walkup, G.,Wei, Y.,Stokes, S. (登録日: 2010-11-18, 公開日: 2011-01-12, 最終更新日: 2024-02-21)

主引用文献

Mills, S.D.,Eakin, A.E.,Buurman, E.T.,Newman, J.V.,Gao, N.,Huynh, H.,Johnson, K.D.,Lahiri, S.,Shapiro, A.B.,Walkup, G.K.,Yang, W.,Stokes, S.S.
Novel Bacterial NAD+-Dependent DNA Ligase Inhibitors with Broad-Spectrum Activity and Antibacterial Efficacy In Vivo.
Antimicrob.Agents Chemother., 55:1088-1096, 2011

PubMed Abstract: DNA ligases are indispensable enzymes playing a critical role in DNA replication, recombination, and repair in all living organisms. Bacterial NAD+-dependent DNA ligase (LigA) was evaluated for its potential as a broad-spectrum antibacterial target. A novel class of substituted adenosine analogs was discovered by target-based high-throughput screening (HTS), and these compounds were optimized to render them more effective and selective inhibitors of LigA. The adenosine analogs inhibited the LigA activities of Escherichia coli, Haemophilus influenzae, Mycoplasma pneumoniae, Streptococcus pneumoniae, and Staphylococcus aureus, with inhibitory activities in the nanomolar range. They were selective for bacterial NAD+-dependent DNA ligases, showing no inhibitory activity against ATP-dependent human DNA ligase 1 or bacteriophage T4 ligase. Enzyme kinetic measurements demonstrated that the compounds bind competitively with NAD+. X-ray crystallography demonstrated that the adenosine analogs bind in the AMP-binding pocket of the LigA adenylation domain. Antibacterial activity was observed against pathogenic Gram-positive and atypical bacteria, such as S. aureus, S. pneumoniae, Streptococcus pyogenes, and M. pneumoniae, as well as against Gram-negative pathogens, such as H. influenzae and Moraxella catarrhalis. The mode of action was verified using recombinant strains with altered LigA expression, an Okazaki fragment accumulation assay, and the isolation of resistant strains with ligA mutations. In vivo efficacy was demonstrated in a murine S. aureus thigh infection model and a murine S. pneumoniae lung infection model. Treatment with the adenosine analogs reduced the bacterial burden (expressed in CFU) in the corresponding infected organ tissue as much as 1,000-fold, thus validating LigA as a target for antibacterial therapy.

PubMed: 21189350
DOI: 10.1128/AAC.01181-10

実験手法

X-RAY DIFFRACTION (2 Å)