3PI0

Endothiapepsin in complex with a fragment

3PI0 の概要

• エントリーDOI: 10.2210/pdb3pi0/pdb
• 関連するPDBエントリー: 3PB5 3PBD 3PBZ 3PCW 3PCZ 3PGI 3PLD 3PLL 3PM4 3PMU 3PMY
• 分子名称: Endothiapepsin, N-(pyridin-4-ylmethyl)-2,3-dihydro-1,4-benzodioxin-6-amine, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Cryphonectria parasitica (Chesnut blight fungus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34318.45

構造登録者

Koester, H.,Heine, A.,Klebe, G. (登録日: 2010-11-05, 公開日: 2011-11-02, 最終更新日: 2024-10-16)

主引用文献

Koster, H.,Craan, T.,Brass, S.,Herhaus, C.,Zentgraf, M.,Neumann, L.,Heine, A.,Klebe, G.
A small nonrule of 3 compatible fragment library provides high hit rate of endothiapepsin crystal structures with various fragment chemotypes.
J.Med.Chem., 54:7784-7796, 2011

PubMed Abstract: Druglike molecules are defined by Lipinski's rule of 5, to characterize fragment thresholds, they have been reduced from 5 to 3 (Astex's rule of 3). They are applied to assemble fragment libraries, and providers use them to select fragments for commercial offer. We question whether these rules are too stringent to compose fragment libraries with candidates exhibiting sufficient room for chemical subsequent growing and merging modifications as appropriate functional groups for chemical transformations are required. Usually these groups exhibit properties as hydrogen bond donors/acceptors and provide entry points for optimization chemistry. We therefore designed a fragment library (364 entries) without strictly applying the rule of 3. For initial screening for endothiapepsin binding, we performed a biochemical cleavage assay of a fluorogenic substrate at 1 mM. "Hits" were defined to inhibit the enzyme by at least 40%. Fifty-five hits were suggested and subsequently soaked into endothiapepsin crystals. Eleven crystal structures could be determined covering fragments with diverse binding modes: (i) direct binding to the catalytic dyad aspartates, (ii) water-mediated binding to the aspartates, (iii) no direct interaction with the dyad. They occupy different specificity pockets. Only 4 of the 11 fragments are consistent with the rule of 3. Restriction to this rule would have limited the fragment hits to a strongly reduced variety of chemotypes.

PubMed: 21972967
DOI: 10.1021/jm200642w

実験手法

X-RAY DIFFRACTION (1.64 Å)