3PE3

Structure of human O-GlcNAc transferase and its complex with a peptide substrate

3PE3 の概要

• エントリーDOI: 10.2210/pdb3pe3/pdb
• 関連するPDBエントリー: 3PE4
• 分子名称: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, URIDINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: gt-b, glycosyltransferase, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: O15294
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 325514.68

構造登録者

Lazarus, M.B.,Nam, Y.,Jiang, J.,Sliz, P.,Walker, S. (登録日: 2010-10-25, 公開日: 2011-01-19, 最終更新日: 2024-02-21)

主引用文献

Lazarus, M.B.,Nam, Y.,Jiang, J.,Sliz, P.,Walker, S.
Structure of human O-GlcNAc transferase and its complex with a peptide substrate.
Nature, 469:564-567, 2011

PubMed Abstract: The essential mammalian enzyme O-linked β-N-acetylglucosamine transferase (O-GlcNAc transferase, here OGT) couples metabolic status to the regulation of a wide variety of cellular signalling pathways by acting as a nutrient sensor. OGT catalyses the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine (UDP-GlcNAc) to serines and threonines of cytoplasmic, nuclear and mitochondrial proteins, including numerous transcription factors, tumour suppressors, kinases, phosphatases and histone-modifying proteins. Aberrant glycosylation by OGT has been linked to insulin resistance, diabetic complications, cancer and neurodegenerative diseases including Alzheimer's. Despite the importance of OGT, the details of how it recognizes and glycosylates its protein substrates are largely unknown. We report here two crystal structures of human OGT, as a binary complex with UDP (2.8 Å resolution) and as a ternary complex with UDP and a peptide substrate (1.95 Å). The structures provide clues to the enzyme mechanism, show how OGT recognizes target peptide sequences, and reveal the fold of the unique domain between the two halves of the catalytic region. This information will accelerate the rational design of biological experiments to investigate OGT's functions; it will also help the design of inhibitors for use as cellular probes and help to assess its potential as a therapeutic target.

PubMed: 21240259
DOI: 10.1038/nature09638

実験手法

X-RAY DIFFRACTION (2.78 Å)