3PCQ

Femtosecond X-ray protein Nanocrystallography

Summary for 3PCQ

Related1JB0
DescriptorPhotosystem I P700 chlorophyll a apoprotein A1, Photosystem I P700 chlorophyll a apoprotein A2, Photosystem I iron-sulfur center, ... (20 entities in total)
Functional Keywordsmembrane protein, multiprotein-pigment complex, photosynthesis
Biological sourceThermosynechococcus elongatus
Cellular locationCellular thylakoid membrane ; Multi- pass membrane protein  P0A405 P0A407 P0A425 Q8DGB4
Cellular thylakoid membrane ; Peripheral membrane protein ; Cytoplasmic side  P0A415
Cellular thylakoid membrane ; Peripheral membrane protein  P0A423
Cellular thylakoid membrane ; Single-pass membrane protein  P0A427 P0A429 P0A403
Total number of polymer chains12
Total molecular weight359828.36
Authors
Chapman, H.N.,Fromme, P.,Barty, A.,White, T.A.,Kirian, R.A.,Aquila, A.,Hunter, M.S.,Schulz, J.,Deponte, D.P.,Weierstall, U.,Doak, R.B.,Maia, F.R.N.C.,Martin, A.V.,Schlichting, I.,Lomb, L.,Coppola, N.,Shoeman, R.L.,Epp, S.W.,Hartmann, R.,Rolles, D.,Rudenko, A.,Foucar, L.,Kimmel, N.,Weidenspointner, G.,Holl, P.,Liang, M.,Barthelmess, M.,Caleman, C.,Boutet, S.,Bogan, M.J.,Krzywinski, J.,Bostedt, C.,Bajt, S.,Gumprecht, L.,Rudek, B.,Erk, B.,Schmidt, C.,Homke, A.,Reich, C.,Pietschner, D.,Struder, L.,Hauser, G.,Gorke, H.,Ullrich, J.,Herrmann, S.,Schaller, G.,Schopper, F.,Soltau, H.,Kuhnel, K.-U.,Messerschmidt, M.,Bozek, J.D.,Hau-Riege, S.P.,Frank, M.,Hampton, C.Y.,Sierra, R.,Starodub, D.,Williams, G.J.,Hajdu, J.,Timneanu, N.,Seibert, M.M.,Andreasson, J.,Rocker, A.,Jonsson, O.,Svenda, M.,Stern, S.,Nass, K.,Andritschke, R.,Schroter, C.-D.,Krasniqi, F.,Bott, M.,Schmidt, K.E.,Wang, X.,Grotjohann, I.,Holton, J.M.,Barends, T.R.M.,Neutze, R.,Marchesini, S.,Fromme, R.,Schorb, S.,Rupp, D.,Adolph, M.,Gorkhover, T.,Andersson, I.,Hirsemann, H.,Potdevin, G.,Graafsma, H.,Nilsson, B.,Spence, J.C.H. (deposition date: 2010-10-21, release date: 2011-02-02, Last modification date: 2018-02-14)
Primary citation
Chapman, H.N.,Fromme, P.,Barty, A.,White, T.A.,Kirian, R.A.,Aquila, A.,Hunter, M.S.,Schulz, J.,Deponte, D.P.,Weierstall, U.,Doak, R.B.,Maia, F.R.,Martin, A.V.,Schlichting, I.,Lomb, L.,Coppola, N.,Shoeman, R.L.,Epp, S.W.,Hartmann, R.,Rolles, D.,Rudenko, A.,Foucar, L.,Kimmel, N.,Weidenspointner, G.,Holl, P.,Liang, M.,Barthelmess, M.,Caleman, C.,Boutet, S.,Bogan, M.J.,Krzywinski, J.,Bostedt, C.,Bajt, S.,Gumprecht, L.,Rudek, B.,Erk, B.,Schmidt, C.,Homke, A.,Reich, C.,Pietschner, D.,Struder, L.,Hauser, G.,Gorke, H.,Ullrich, J.,Herrmann, S.,Schaller, G.,Schopper, F.,Soltau, H.,Kuhnel, K.U.,Messerschmidt, M.,Bozek, J.D.,Hau-Riege, S.P.,Frank, M.,Hampton, C.Y.,Sierra, R.G.,Starodub, D.,Williams, G.J.,Hajdu, J.,Timneanu, N.,Seibert, M.M.,Andreasson, J.,Rocker, A.,Jonsson, O.,Svenda, M.,Stern, S.,Nass, K.,Andritschke, R.,Schroter, C.D.,Krasniqi, F.,Bott, M.,Schmidt, K.E.,Wang, X.,Grotjohann, I.,Holton, J.M.,Barends, T.R.,Neutze, R.,Marchesini, S.,Fromme, R.,Schorb, S.,Rupp, D.,Adolph, M.,Gorkhover, T.,Andersson, I.,Hirsemann, H.,Potdevin, G.,Graafsma, H.,Nilsson, B.,Spence, J.C.
Femtosecond X-ray protein nanocrystallography.
Nature, 470:73-77, 2011
PubMed: 21293373 (PDB entries with the same primary citation)
DOI: 10.1038/nature09750
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (8.984 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.228151.7%4.4%3.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 3pcq
no rotation
Molmil generated image of 3pcq
rotated about x axis by 90°
Molmil generated image of 3pcq
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 3pcq
no rotation
Molmil generated image of 3pcq
rotated about x axis by 90°
Molmil generated image of 3pcq
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (3pcq.pdb1.gz [1.41 MB])