3P6B

The crystal structure of CelK CBM4 from Clostridium thermocellum

Summary for 3P6B

• Entry DOI: 10.2210/pdb3p6b/pdb
• Related: 3K4Z
• Descriptor: Cellulose 1,4-beta-cellobiosidase, CALCIUM ION, ACETATE ION, ... (6 entities in total)
• Functional Keywords: beta-sandwich, carbohydrate binding, sugar binding protein
• Biological source: Clostridium thermocellum
• Cellular location: Secreted: P0C2S1
• Total number of polymer chains: 2
• Total formula weight: 48460.65

Authors

Alahuhta, P.M.,Luo, Y.,Lunin, V.V. (deposition date: 2010-10-11, release date: 2011-08-24, Last modification date: 2023-09-06)

Primary citation

Alahuhta, M.,Luo, Y.,Ding, S.Y.,Himmel, M.E.,Lunin, V.V.
Structure of CBM4 from Clostridium thermocellum cellulase K.
Acta Crystallogr.,Sect.F, 67:527-530, 2011

PubMed Abstract: Here, a 2.0 Å resolution X-ray structure of Clostridium thermocellum cellulase K family 4 carbohydrate-binding module (CelK CBM4) is reported. The resulting structure was refined to an R factor of 0.212 and an R(free) of 0.274. Structural analysis shows that this new structure is very similar to the previously solved structure of C. thermocellum CbhA CBM4. Most importantly, these data support the previously proposed notion of an extended binding pocket using a novel tryptophan-containing loop that may be highly conserved in clostridial CBM4 proteins.

PubMed: 21543854
DOI: 10.1107/S1744309111003307

Experimental method

X-RAY DIFFRACTION (2 Å)