3OPI

7-DEAZA-2'-DEOXYADENOSINE modification in B-FORM DNA

Summary for 3OPI

• Entry DOI: 10.2210/pdb3opi/pdb
• Descriptor: DNA (5'-D(*CP*GP*CP*GP*AP*(7DA)P*TP*TP*CP*GP*CP*G)-3'), MAGNESIUM ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: b-dna, dodecamer, 7-deaza-deoxyadenosine, 7-deaza-da, dna
• Total number of polymer chains: 2
• Total formula weight: 7441.07

Authors

Kowal, E.A.,Ganguly, M.,Pallan, P.S.,Marky, L.A.,Gold, B.,Egli, M.,Stone, M.P. (deposition date: 2010-09-01, release date: 2011-08-31, Last modification date: 2023-09-06)

Primary citation

Kowal, E.A.,Ganguly, M.,Pallan, P.S.,Marky, L.A.,Gold, B.,Egli, M.,Stone, M.P.
Altering the Electrostatic Potential in the Major Groove: Thermodynamic and Structural Characterization of 7-Deaza-2'-deoxyadenosine:dT Base Pairing in DNA.
J.Phys.Chem.B, 115:13925-13934, 2011

PubMed Abstract: As part of an ongoing effort to explore the effect of major groove electrostatics on the thermodynamic stability and structure of DNA, a 7-deaza-2'-deoxyadenosine:dT (7-deaza-dA:dT) base pair in the Dickerson-Drew dodecamer (DDD) was studied. The removal of the electronegative N7 atom on dA and the replacement with an electropositive C-H in the major groove was expected to have a significant effect on major groove electrostatics. The structure of the 7-deaza-dA:dT base pair was determined at 1.1 Å resolution in the presence of Mg(2+). The 7-deaza-dA, which is isosteric for dA, had minimal effect on the base pairing geometry and the conformation of the DDD in the crystalline state. There was no major groove cation association with the 7-deaza-dA heterocycle. In solution, circular dichroism showed a positive Cotton effect centered at 280 nm and a negative Cotton effect centered at 250 nm that were characteristic of a right-handed helix in the B-conformation. However, temperature-dependent NMR studies showed increased exchange between the thymine N3 imino proton of the 7-deaza-dA:dT base pair and water, suggesting reduced stacking interactions and an increased rate of base pair opening. This correlated with the observed thermodynamic destabilization of the 7-deaza-dA modified duplex relative to the DDD. A combination of UV melting and differential scanning calorimetry experiments were conducted to evaluate the relative contributions of enthalpy and entropy in the thermodynamic destabilization of the DDD. The most significant contribution arose from an unfavorable enthalpy term, which probably results from less favorable stacking interactions in the modified duplex, which was accompanied by a significant reduction in the release of water and cations from the 7-deaza-dA modified DNA.

PubMed: 22059929
DOI: 10.1021/jp207104w

Experimental method

X-RAY DIFFRACTION (1.1 Å)