3OFS
Dynamic conformations of the CD38-mediated NAD cyclization captured using multi-copy crystallography
Summary for 3OFS
Entry DOI | 10.2210/pdb3ofs/pdb |
Related | 3I9M |
Descriptor | ADP-ribosyl cyclase 1, [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl [(2R,3R,4R)-4-fluoro-3-hydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate (3 entities in total) |
Functional Keywords | cd38, cyclization, hydrolysis, cadpr, adpr, ara-2'f-nad, ara-2'f-adpr, hydrolase |
Biological source | Homo sapiens (human) |
Cellular location | Membrane; Single-pass type II membrane protein: P28907 |
Total number of polymer chains | 6 |
Total formula weight | 181800.06 |
Authors | |
Primary citation | Zhang, H.,Graeff, R.,Chen, Z.,Zhang, L.R.,Zhang, L.H.,Lee, H.C.,Hao, Q. Dynamic Conformations of the CD38-Mediated NAD Cyclization Captured in a Single Crystal J.Mol.Biol., 405:1070-1078, 2011 Cited by PubMed Abstract: The extracellular domain of human CD38 is a multifunctional enzyme involved in the metabolism of two Ca(2+) messengers: cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate. When NAD is used as substrate, CD38 predominantly hydrolyzes it to ADP-ribose, with a trace amount of cyclic ADP-ribose produced through cyclization of the substrate. However, mutation of a key residue at the active site, E146, inhibits the hydrolysis activity of CD38 but greatly increases its cyclization activity. To understand the role of the residue E146 in the catalytic process, we determined the crystal structure of the E146A mutant protein with a substrate analogue, arabinosyl-2'-fluoro-deoxy-nicotinamide adenine dinucleotide. The structure captured the enzymatic reaction intermediates in six different conformations in a crystallographic asymmetric unit. The structural results indicate a folding-back process for the adenine ring of the substrate and provide the first multiple snapshots of the process. Our approach of utilizing multiple molecules in the crystallographic asymmetric unit should be generally applicable for capturing the dynamic nature of enzymatic catalysis. PubMed: 21134381DOI: 10.1016/j.jmb.2010.11.044 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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