3OFS

Dynamic conformations of the CD38-mediated NAD cyclization captured using multi-copy crystallography

3OFS の概要

• エントリーDOI: 10.2210/pdb3ofs/pdb
• 関連するPDBエントリー: 3I9M
• 分子名称: ADP-ribosyl cyclase 1, [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl [(2R,3R,4R)-4-fluoro-3-hydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate (3 entities in total)
• 機能のキーワード: cd38, cyclization, hydrolysis, cadpr, adpr, ara-2'f-nad, ara-2'f-adpr, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type II membrane protein: P28907
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 181800.06

構造登録者

Zhang, H.,Lee, H.C.,Hao, Q. (登録日: 2010-08-16, 公開日: 2010-12-15, 最終更新日: 2024-11-06)

主引用文献

Zhang, H.,Graeff, R.,Chen, Z.,Zhang, L.R.,Zhang, L.H.,Lee, H.C.,Hao, Q.
Dynamic Conformations of the CD38-Mediated NAD Cyclization Captured in a Single Crystal
J.Mol.Biol., 405:1070-1078, 2011

PubMed Abstract: The extracellular domain of human CD38 is a multifunctional enzyme involved in the metabolism of two Ca(2+) messengers: cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate. When NAD is used as substrate, CD38 predominantly hydrolyzes it to ADP-ribose, with a trace amount of cyclic ADP-ribose produced through cyclization of the substrate. However, mutation of a key residue at the active site, E146, inhibits the hydrolysis activity of CD38 but greatly increases its cyclization activity. To understand the role of the residue E146 in the catalytic process, we determined the crystal structure of the E146A mutant protein with a substrate analogue, arabinosyl-2'-fluoro-deoxy-nicotinamide adenine dinucleotide. The structure captured the enzymatic reaction intermediates in six different conformations in a crystallographic asymmetric unit. The structural results indicate a folding-back process for the adenine ring of the substrate and provide the first multiple snapshots of the process. Our approach of utilizing multiple molecules in the crystallographic asymmetric unit should be generally applicable for capturing the dynamic nature of enzymatic catalysis.

PubMed: 21134381
DOI: 10.1016/j.jmb.2010.11.044

実験手法

X-RAY DIFFRACTION (2.2 Å)