3O7X

Crystal structure of human Hili PAZ domain

3O7X の概要

• エントリーDOI: 10.2210/pdb3o7x/pdb
• 関連するPDBエントリー: 3O3I 3O6E 3O7V
• 分子名称: Piwi-like protein 2 (2 entities in total)
• 機能のキーワード: piwi, rna silencing, pi-rna, hiwi1, hili, paz domain, rna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm : Q8TC59
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 65686.85

構造登録者

Tian, Y.,Simanshu, D.K.,Ma, J.-B.,Patel, D.J. (登録日: 2010-08-01, 公開日: 2011-01-12, 最終更新日: 2024-02-21)

主引用文献

Tian, Y.,Simanshu, D.K.,Ma, J.B.,Patel, D.J.
Inaugural Article: Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.
Proc.Natl.Acad.Sci.USA, 108:903-910, 2011

PubMed Abstract: Argonaute and Piwi proteins are key players in the RNA silencing pathway, with the former interacting with micro-RNAs (miRNAs) and siRNAs, whereas the latter targets piwi-interacting RNAs (piRNAs) that are 2'-O-methylated (2(')-OCH(3)) at their 3' ends. Germline-specific piRNAs and Piwi proteins play a critical role in genome defense against transposable elements, thereby protecting the genome against transposon-induced defects in gametogenesis and fertility. Humans contain four Piwi family proteins designated Hiwi1, Hiwi2, Hiwi3, and Hili. We report on the structures of Hili-PAZ (Piwi/Argonaute/Zwille) domain in the free state and Hiwi1 PAZ domain bound to self-complementary 14-mer RNAs (12-bp + 2-nt overhang) containing 2(')-OCH(3) and 2'-OH at their 3' ends. These structures explain the molecular basis underlying accommodation of the 2(')-OCH(3) group within a preformed Hiwi1 PAZ domain binding pocket, whose hydrophobic characteristics account for the preferential binding of 2(')-OCH(3) over 2'-OH 3' ends. These results contrast with the more restricted binding pocket for the human Ago1 PAZ domain, which exhibits a reverse order, with preferential binding of 2'-OH over 2(')-OCH(3) 3' ends.

PubMed: 21193640
DOI: 10.1073/pnas.1017762108

実験手法

X-RAY DIFFRACTION (2.9248 Å)