3O7X
Crystal structure of human Hili PAZ domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.535, 142.949, 226.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.385 - 2.925 |
| R-factor | 0.2506 |
| Rwork | 0.246 |
| R-free | 0.29340 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.887 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.4_153) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.020 |
| High resolution limit [Å] | 2.920 | 6.290 | 2.920 |
| Rmerge | 0.091 | 0.053 | 0.307 |
| Number of reflections | 14092 | ||
| <I/σ(I)> | 20.2 | 3 | |
| Completeness [%] | 95.1 | 99.7 | 67.2 |
| Redundancy | 6.9 | 6.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 4-8% PEG4000 and 50 mM MgSO4 in 50 mM MES buffer, pH 5.6 to 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
