3O62

Nucleosome core particle modified with a cisplatin 1,3-cis-{Pt(NH3)2}2+-d(GpTpG) intrastrand cross-link

3O62 の概要

• エントリーDOI: 10.2210/pdb3o62/pdb
• 分子名称: Histone H3.2, Histone H4, Histone H2A type 1, ... (7 entities in total)
• 機能のキーワード: nucleosome, cisplatin, structural protein-dna complex, structural protein/dna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• 細胞内の位置: Nucleus: P84233 P62799 P06897 P02281
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 198407.88

構造登録者

Lippard, S.J.,Todd, R.C. (登録日: 2010-07-28, 公開日: 2011-01-05, 最終更新日: 2024-02-21)

主引用文献

Todd, R.C.,Lippard, S.J.
Consequences of Cisplatin binding on nucleosome structure and dynamics.
Chem.Biol., 17:1334-1343, 2010

PubMed Abstract: The effects of cisplatin binding to DNA were explored at the nucleosome level to incorporate key features of the eukaryotic nuclear environment. An X-ray crystal structure of a site-specifically platinated nucleosome carrying a 1,3-cis-{Pt(NH₃)₂}²+-d(GpTpG) intrastrand cross-link reveals the details of how this adduct dictates the rotational positioning of DNA in the nucleosome. Results from in vitro nucleosome mobility assays indicate that a single platinum adduct interferes with ATP-independent sliding of DNA around the octamer core. Data from in vitro transcription experiments suggest that RNA polymerases can successfully navigate along cisplatin-damaged DNA templates that contain nucleosomes, but stall when the transcription elongation complex physically contacts a platinum cross-link located on the template strand. These results provide information about the effects of cisplatin binding to nuclear DNA and enhance our understanding of the mechanism of transcription inhibition by platinum anticancer compounds.

PubMed: 21168769
DOI: 10.1016/j.chembiol.2010.10.018

実験手法

X-RAY DIFFRACTION (3.216 Å)