3O4P

DFPase at 0.85 Angstrom resolution (H atoms included)

3O4P の概要

• エントリーDOI: 10.2210/pdb3o4p/pdb
• 関連するPDBエントリー: 1PJX
• 分子名称: Diisopropyl-fluorophosphatase, CALCIUM ION, GLYCEROL, ... (11 entities in total)
• 機能のキーワード: beta-propeller, hydrolase
• 由来する生物種: Loligo vulgaris (Common European squid)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37265.29

構造登録者

Liebschner, D.,Elias, M.,Koepke, J.,Lecomte, C.,Guillot, B.,Jelsch, C.,Chabriere, E. (登録日: 2010-07-27, 公開日: 2011-08-17, 最終更新日: 2024-04-03)

主引用文献

Elias, M.,Liebschner, D.,Koepke, J.,Lecomte, C.,Guillot, B.,Jelsch, C.,Chabriere, E.
Hydrogen atoms in protein structures: high-resolution X-ray diffraction structure of the DFPase.
BMC Res Notes, 6:308-308, 2013

PubMed Abstract: Hydrogen atoms represent about half of the total number of atoms in proteins and are often involved in substrate recognition and catalysis. Unfortunately, X-ray protein crystallography at usual resolution fails to access directly their positioning, mainly because light atoms display weak contributions to diffraction. However, sub-Ångstrom diffraction data, careful modeling and a proper refinement strategy can allow the positioning of a significant part of hydrogen atoms.

PubMed: 23915572
DOI: 10.1186/1756-0500-6-308

実験手法

X-RAY DIFFRACTION (0.85 Å)