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3O2P

A Dual E3 Mechanism for Rub1 Ligation to Cdc53: Dcn1(P)-Cdc53(WHB)

3O2P の概要
エントリーDOI10.2210/pdb3o2p/pdb
関連するPDBエントリー3O2U 3O6B
分子名称Defective in cullin neddylation protein 1, Cell division control protein 53, GLYCEROL, ... (4 entities in total)
機能のキーワードligase, cell cycle
由来する生物種Saccharomyces cerevisiae (yeast)
詳細
細胞内の位置Cytoplasm : Q12018
タンパク質・核酸の鎖数2
化学式量合計34799.57
構造登録者
Scott, D.C.,Monda, J.K.,Grace, C.R.R.,Duda, D.M.,Kriwacki, R.W.,Kurz, T.,Schulman, B.A. (登録日: 2010-07-22, 公開日: 2010-09-15, 最終更新日: 2023-09-06)
主引用文献Scott, D.C.,Monda, J.K.,Grace, C.R.,Duda, D.M.,Kriwacki, R.W.,Kurz, T.,Schulman, B.A.
A dual E3 mechanism for Rub1 ligation to Cdc53.
Mol.Cell, 39:784-796, 2010
Cited by
PubMed Abstract: In ubiquitin-like protein (UBL) cascades, a thioester-linked E2∼UBL complex typically interacts with an E3 enzyme for UBL transfer to the target. Here we demonstrate a variant mechanism, whereby the E2 Ubc12 functions with two E3s, Hrt1 and Dcn1, for ligation of the UBL Rub1 to Cdc53's WHB subdomain. Hrt1 functions like a conventional RING E3, with its N terminus recruiting Cdc53 and C-terminal RING activating Ubc12∼Rub1. Dcn1's "potentiating neddylation" domain (Dcn1(P)) acts as an additional E3, reducing nonspecific Hrt1-mediated Ubc12∼Rub1 discharge and directing Ubc12's active site to Cdc53. Crystal structures of Dcn1(P)-Cdc53(WHB) and Ubc12 allow modeling of a catalytic complex, supported by mutational data. We propose that Dcn1's interactions with both Cdc53 and Ubc12 would restrict the otherwise flexible Hrt1 RING-bound Ubc12∼Rub1 to a catalytically competent orientation. Our data reveal mechanisms by which two E3s function synergistically to promote UBL transfer from one E2 to a target.
PubMed: 20832729
DOI: 10.1016/j.molcel.2010.08.030
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.233 Å)
構造検証レポート
Validation report summary of 3o2p
検証レポート(詳細版)ダウンロードをダウンロード

239803

件を2025-08-06に公開中

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