3O0I

Structure of the human Hsp90-alpha N-domain bound to the hsp90 inhibitor PU-H54

3O0I の概要

• エントリーDOI: 10.2210/pdb3o0i/pdb
• 関連するPDBエントリー: 3C11
• 分子名称: HSP90AA1 protein, 8-[(2,4-dimethylphenyl)sulfanyl]-3-pent-4-yn-1-yl-3H-purin-6-amine (3 entities in total)
• 機能のキーワード: hsp90 heat-shock proteins, chaperone-inhibitor complex, chaperone/inhibitor
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29110.59

構造登録者

Seidler, P.M.,Gewirth, D.T. (登録日: 2010-07-19, 公開日: 2011-10-05, 最終更新日: 2024-02-21)

主引用文献

Patel, P.D.,Yan, P.,Seidler, P.M.,Patel, H.J.,Sun, W.,Yang, C.,Que, N.S.,Taldone, T.,Finotti, P.,Stephani, R.A.,Gewirth, D.T.,Chiosis, G.
Paralog-selective Hsp90 inhibitors define tumor-specific regulation of HER2.
Nat.Chem.Biol., 9:677-684, 2013

PubMed Abstract: Although the Hsp90 chaperone family, comprised in humans of four paralogs, Hsp90α, Hsp90β, Grp94 and Trap-1, has important roles in malignancy, the contribution of each paralog to the cancer phenotype is poorly understood. This is in large part because reagents to study paralog-specific functions in cancer cells have been unavailable. Here we combine compound library screening with structural and computational analyses to identify purine-based chemical tools that are specific for Hsp90 paralogs. We show that Grp94 selectivity is due to the insertion of these compounds into a new allosteric pocket. We use these tools to demonstrate that cancer cells use individual Hsp90 paralogs to regulate a client protein in a tumor-specific manner and in response to proteome alterations. Finally, we provide new mechanistic evidence explaining why selective Grp94 inhibition is particularly efficacious in certain breast cancers.

PubMed: 23995768
DOI: 10.1038/nchembio.1335

実験手法

X-RAY DIFFRACTION (1.47 Å)