3NWY

Structure and allosteric regulation of the uridine monophosphate kinase from Mycobacterium tuberculosis

3NWY の概要

• エントリーDOI: 10.2210/pdb3nwy/pdb
• 分子名称: Uridylate kinase, GUANOSINE-5'-TRIPHOSPHATE, URIDINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: allosterically activated form, aak fold, ump kinase, transferase
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Cytoplasm (By similarity): P65929
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 181298.60

構造登録者

Labesse, G.,Munier-Lehmann, H. (登録日: 2010-07-12, 公開日: 2010-08-25, 最終更新日: 2024-04-03)

主引用文献

Labesse, G.,Benkali, K.,Salard-Arnaud, I.,Gilles, A.M.,Munier-Lehmann, H.
Structural and functional characterization of the Mycobacterium tuberculosis uridine monophosphate kinase: insights into the allosteric regulation.
Nucleic Acids Res., 39:3458-3472, 2011

PubMed Abstract: Nucleoside Monophosphate Kinases (NMPKs) family are key enzymes in nucleotide metabolism. Bacterial UMPKs depart from the main superfamily of NMPKs. Having no eukaryotic counterparts they represent attractive therapeutic targets. They are regulated by GTP and UTP, while showing different mechanisms in Gram(+), Gram(-) and archaeal bacteria. In this work, we have characterized the mycobacterial UMPK (UMPKmt) combining enzymatic and structural investigations with site-directed mutagenesis. UMPKmt exhibits cooperativity toward ATP and an allosteric regulation by GTP and UTP. The crystal structure of the complex of UMPKmt with GTP solved at 2.5 Å, was merely identical to the modelled apo-form, in agreement with SAXS experiments. Only a small stretch of residues was affected upon nucleotide binding, pointing out the role of macromolecular dynamics rather than major structural changes in the allosteric regulation of bacterial UMPKs. We further probe allosteric regulation by site-directed mutagenesis. In particular, a key residue involved in the allosteric regulation of this enzyme was identified.

PubMed: 21149268
DOI: 10.1093/nar/gkq1250

実験手法

X-RAY DIFFRACTION (2.54 Å)