3N7Q

Crystal structure of human mitochondrial mTERF fragment (aa 99-399) in complex with a 12-mer DNA encompassing the tRNALeu(UUR) binding sequence

Summary for 3N7Q

• Entry DOI: 10.2210/pdb3n7q/pdb
• Related: 3N6S
• Descriptor: Transcription termination factor, mitochondrial, DNA (5'-D(*GP*GP*CP*AP*GP*AP*GP*CP*CP*CP*GP*G)-3'), DNA (5'-D(*CP*CP*GP*GP*GP*CP*TP*CP*TP*GP*CP*C)-3'), ... (5 entities in total)
• Functional Keywords: mitochondrial transcription termination factor-dna complex, mitochondrial replication pausing-dna complex, left-handed helical tandem repeat, protein-dna complex, transcription, replication-dna complex, replication/dna
• Biological source: Homo sapiens (human)
• Cellular location: Mitochondrion: Q99551
• Total number of polymer chains: 3
• Total formula weight: 43227.21

Authors

Jimenez-Menendez, N.,Fernandez-Millan, P.,Rubio-Cosials, A.,Arnan, C.,Montoya, J.,Jacobs, H.T.,Bernado, P.,Coll, M.,Uson, I.,Sola, M. (deposition date: 2010-05-27, release date: 2010-06-16, Last modification date: 2024-02-21)

Primary citation

Jimenez-Menendez, N.,Fernandez-Millan, P.,Rubio-Cosials, A.,Arnan, C.,Montoya, J.,Jacobs, H.T.,Bernado, P.,Coll, M.,Uson, I.,Sola, M.
Human mitochondrial mTERF wraps around DNA through a left-handed superhelical tandem repeat.
Nat.Struct.Mol.Biol., 17:891-893, 2010

PubMed Abstract: The regulation of mitochondrial DNA (mtDNA) processes is slowly being characterized at a structural level. We present here crystal structures of human mitochondrial regulator mTERF, a transcription termination factor also implicated in replication pausing, in complex with double-stranded DNA oligonucleotides containing the tRNA(Leu)(UUR) gene sequence. mTERF comprises nine left-handed helical tandem repeats that form a left-handed superhelix, the Zurdo domain.

PubMed: 20543826
DOI: 10.1038/nsmb.1859

Experimental method

X-RAY DIFFRACTION (2.4 Å)