3N25
The structure of muscle pyruvate kinase in complex with proline, pyruvate, and Mn2+
Summary for 3N25
| Entry DOI | 10.2210/pdb3n25/pdb |
| Descriptor | Pyruvate kinase isozymes M1/M2, PROLINE, MANGANESE (II) ION, ... (9 entities in total) |
| Functional Keywords | pyruvate kinase, glycolysis, allosteric regulation, transferase |
| Biological source | Oryctolagus cuniculus (European rabbit,Japanese white rabbit,domestic rabbit,rabbits) |
| Cellular location | Cytoplasm : P11974 |
| Total number of polymer chains | 8 |
| Total formula weight | 470150.38 |
| Authors | Fenton, A.W.,Johnson, T.A.,Holyoak, T. (deposition date: 2010-05-17, release date: 2010-07-28, Last modification date: 2023-11-15) |
| Primary citation | Fenton, A.W.,Johnson, T.A.,Holyoak, T. The pyruvate kinase model system, a cautionary tale for the use of osmolyte perturbations to support conformational equilibria in allostery. Protein Sci., 19:1796-1800, 2010 Cited by PubMed Abstract: In the study of rabbit muscle pyruvate kinase (M1-PYK), proline has previously been used as an osmolyte in an attempt to determine a role for preexisting conformational equilibria in allosteric regulation. In this context, osmolytes are small molecules assumed to have no direct interaction with the protein. In contrast to proline's proposed role as an osmolyte, the structure of M1PYK-Mn-pyruvate-proline complex reported herein demonstrates that proline binds specifically to the allosteric site of M1-PYK. Therefore, this amino acid is an allosteric effector rather than a benign osmolyte. Other compounds often used as osmolytes (polyethyleneglycol and glycerol) are also present in the structure, suggesting an interaction with the protein that would, in turn, prevent the usefulness of these compounds in the study of this and most likely other proteins. These findings highlight the need to verify that compounds used as osmolytes to perturb preexisting conformational equilibrium do not directly interact with the protein, a consideration not commonly addressed in the past. PubMed: 20629175DOI: 10.1002/pro.450 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.41 Å) |
Structure validation
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