3N0C
TM0449 mutant crystal grown by hanging drop method
Summary for 3N0C
| Entry DOI | 10.2210/pdb3n0c/pdb |
| Related | 3MZQ 3MZR 3N0B 3NO2 3NO3 |
| Descriptor | Thymidylate synthase thyX, 2'-DEOXYURIDINE 5'-MONOPHOSPHATE, FLAVIN-ADENINE DINUCLEOTIDE, ... (4 entities in total) |
| Functional Keywords | crystals in loops, thyx, fdts, transferase |
| Biological source | Thermotoga maritima |
| Total number of polymer chains | 4 |
| Total formula weight | 113624.74 |
| Authors | Mathews, I.I. (deposition date: 2010-05-13, release date: 2011-05-25, Last modification date: 2023-09-06) |
| Primary citation | Berger, M.A.,Decker, J.H.,Mathews, I.I. Diffraction study of protein crystals grown in cryoloops and micromounts. J.Appl.Crystallogr., 43:1513-1518, 2010 Cited by PubMed Abstract: Protein crystals are usually grown in hanging or sitting drops and generally get transferred to a loop or micromount for cryocooling and data collection. This paper describes a method for growing crystals on cryoloops for easier manipulation of the crystals for data collection. This study also investigates the steps for the automation of this process and describes the design of a new tray for the method. The diffraction patterns and the structures of three proteins grown by both the new method and the conventional hanging-drop method are compared. The new setup is optimized for the automation of the crystal mounting process. Researchers could prepare nanolitre drops under ordinary laboratory conditions by growing the crystals directly in loops or micromounts. As has been pointed out before, higher levels of supersaturation can be obtained in very small volumes, and the new method may help in the exploration of additional crystallization conditions. PubMed: 22477781DOI: 10.1107/S0021889810040409 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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