3N0C
TM0449 mutant crystal grown by hanging drop method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.549, 116.718, 141.202 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.19585 |
| Rwork | 0.193 |
| R-free | 0.24754 |
| Structure solution method | Rigid body refinemet |
| Starting model (for MR) | 3nob |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.832 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.089 | 0.520 |
| Number of reflections | 40698 | |
| <I/σ(I)> | 12.8 | 2.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 295 | 49% PEG 200, 0.1M HEPES (pH 7.6), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
