3MU0

Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region A of the crystal. Third step of radiation damage

3MU0 の概要

• エントリーDOI: 10.2210/pdb3mu0/pdb
• 関連するPDBエントリー: 1GVK 3MTY 3MU1 3MU4 3MU5 3MU8
• 分子名称: Chymotrypsin-like elastase family member 1, SODIUM ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: radiation damage, disulfide bridge, atomic resolution, hydrolase
• 由来する生物種: Sus scrofa (pigs,swine,wild boar)
• 細胞内の位置: Secreted: P00772
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26143.15

構造登録者

Petrova, T.,Ginell, S.,Mitschler, A.,Cousido-Siah, A.,Hazemann, I.,Podjarny, A.,Joachimiak, A. (登録日: 2010-05-01, 公開日: 2010-05-12, 最終更新日: 2023-09-06)

主引用文献

Petrova, T.,Ginell, S.,Mitschler, A.,Kim, Y.,Lunin, V.Y.,Joachimiak, G.,Cousido-Siah, A.,Hazemann, I.,Podjarny, A.,Lazarski, K.,Joachimiak, A.
X-ray-induced deterioration of disulfide bridges at atomic resolution.
Acta Crystallogr.,Sect.D, 66:1075-1091, 2010

PubMed Abstract: Overall and site-specific X-ray-induced damage to porcine pancreatic elastase was studied at atomic resolution at temperatures of 100 and 15 K. The experiments confirmed that irradiation causes small movements of protein domains and bound water molecules in protein crystals. These structural changes occur not only at 100 K but also at temperatures as low as 15 K. An investigation of the deterioration of disulfide bridges demonstrated the following. (i) A decrease in the occupancy of S(γ) atoms and the appearance of new cysteine rotamers occur simultaneously. (ii) The occupancy decrease is observed for all S(γ) atoms, while new rotamers arise for some of the cysteine residues; the appearance of new conformations correlates with the accessibility to solvent. (iii) The sum of the occupancies of the initial and new conformations of a cysteine residue is approximately equal to the occupancy of the second cysteine residue in the bridge. (iv) The most pronounced changes occur at doses below 1.4 × 10(7) Gy, with only small changes occurring at higher doses. Comparison of the radiation-induced changes in an elastase crystal at 100 and 15 K suggested that the dose needed to induce a similar level of deterioration of the disulfide bonds and atomic displacements at 15 K to those seen at 100 K is more than two times higher.

PubMed: 20944241
DOI: 10.1107/S0907444910033986
主引用文献が同じPDBエントリー

実験手法

X-RAY DIFFRACTION (1.401 Å)