3MP7

Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes

3MP7 の概要

• エントリーDOI: 10.2210/pdb3mp7/pdb
• 関連するPDBエントリー: 1RH5 2YXQ 2YXR 2ZJS 3DKN
• 分子名称: Preprotein translocase subunit secY, Preprotein translocase subunit secE (2 entities in total)
• 機能のキーワード: protein transport, membrane protein complex, preprotein translocase, membrane insertion, structural genomics, psi-2, protein structure initiative, center for structures of membrane proteins, csmp
• 由来する生物種: Pyrococcus furiosus; 詳細
• 細胞内の位置: Cell membrane ; Multi-pass membrane protein : Q8U019; Cell membrane ; Single-pass membrane protein : Q8TZK2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60883.81

構造登録者

Egea, P.F.,Stroud, R.M.,Center for Structures of Membrane Proteins (CSMP) (登録日: 2010-04-26, 公開日: 2010-10-06, 最終更新日: 2023-09-06)

主引用文献

Egea, P.F.,Stroud, R.M.
Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes.
Proc.Natl.Acad.Sci.USA, 107:17182-17187, 2010

PubMed Abstract: The structure of the protein-translocating channel SecYEβ from Pyrococcus furiosus at 3.1-Å resolution suggests a mechanism for chaperoning transmembrane regions of a protein substrate during its lateral delivery into the lipid bilayer. Cytoplasmic segments of SecY orient the C-terminal α-helical region of another molecule, suggesting a general binding mode and a promiscuous guiding surface capable of accommodating diverse nascent chains at the exit of the ribosomal tunnel. To accommodate this putative nascent chain mimic, the cytoplasmic vestibule widens, and a lateral exit portal is opened throughout its entire length for partition of transmembrane helical segments to the lipid bilayer. In this primed channel, the central plug still occludes the pore while the lateral gate is opened, enabling topological arbitration during early protein insertion. In vivo, a 15 amino acid truncation of the cytoplasmic C-terminal helix of SecY fails to rescue a secY-deficient strain, supporting the essential role of this helix as suggested from the structure.

PubMed: 20855604
DOI: 10.1073/pnas.1012556107

実験手法

X-RAY DIFFRACTION (2.9 Å)