3MOR

Crystal structure of Cathepsin B from Trypanosoma Brucei

Summary for 3MOR

• Entry DOI: 10.2210/pdb3mor/pdb
• Related: 1HUC 1QDQ
• Descriptor: Cathepsin B-like cysteine protease, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, GLYCEROL, ... (6 entities in total)
• Functional Keywords: thiol protease, hydrolase
• Biological source: Trypanosoma brucei
• Total number of polymer chains: 2
• Total formula weight: 71246.38

Authors

Cupelli, K.,Stehle, T. (deposition date: 2010-04-23, release date: 2011-11-02, Last modification date: 2024-11-06)

Primary citation

Koopmann, R.,Cupelli, K.,Redecke, L.,Nass, K.,Deponte, D.P.,White, T.A.,Stellato, F.,Rehders, D.,Liang, M.,Andreasson, J.,Aquila, A.,Bajt, S.,Barthelmess, M.,Barty, A.,Bogan, M.J.,Bostedt, C.,Boutet, S.,Bozek, J.D.,Caleman, C.,Coppola, N.,Davidsson, J.,Doak, R.B.,Ekeberg, T.,Epp, S.W.,Erk, B.,Fleckenstein, H.,Foucar, L.,Graafsma, H.,Gumprecht, L.,Hajdu, J.,Hampton, C.Y.,Hartmann, A.,Hartmann, R.,Hauser, G.,Hirsemann, H.,Holl, P.,Hunter, M.S.,Kassemeyer, S.,Kirian, R.A.,Lomb, L.,Maia, F.R.,Kimmel, N.,Martin, A.V.,Messerschmidt, M.,Reich, C.,Rolles, D.,Rudek, B.,Rudenko, A.,Schlichting, I.,Schulz, J.,Seibert, M.M.,Shoeman, R.L.,Sierra, R.G.,Soltau, H.,Stern, S.,Struder, L.,Timneanu, N.,Ullrich, J.,Wang, X.,Weidenspointner, G.,Weierstall, U.,Williams, G.J.,Wunderer, C.B.,Fromme, P.,Spence, J.C.,Stehle, T.,Chapman, H.N.,Betzel, C.,Duszenko, M.
In vivo protein crystallization opens new routes in structural biology.
Nat.Methods, 9:259-262, 2012

PubMed Abstract: Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

PubMed: 22286384
DOI: 10.1038/nmeth.1859

Experimental method

X-RAY DIFFRACTION (2.55 Å)