3ML5

Crystal structure of the C183S/C217S mutant of human CA VII in complex with acetazolamide

3ML5 の概要

• エントリーDOI: 10.2210/pdb3ml5/pdb
• 分子名称: Carbonic anhydrase 7, ZINC ION, 5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE, ... (4 entities in total)
• 機能のキーワード: protein-inhibitor complex, lyase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm (Probable): P43166
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30396.38

構造登録者

Di Fiore, A.,Truppo, E.,Supuran, C.T.,Alterio, V.,Dathan, N.,Bootorabi, F.,Parkkila, S.,Monti, S.M.,De Simone, G. (登録日: 2010-04-16, 公開日: 2011-03-02, 最終更新日: 2024-10-09)

主引用文献

Di Fiore, A.,Truppo, E.,Supuran, C.T.,Alterio, V.,Dathan, N.,Bootorabi, F.,Parkkila, S.,Monti, S.M.,De Simone, G.
Crystal structure of the C183S/C217S mutant of human CA VII in complex with acetazolamide
Bioorg.Med.Chem.Lett., 20:5023-5026, 2010

PubMed Abstract: Human carbonic anhydrase VII (hCA VII) is a cytosolic member of the alpha-CA family. This enzyme is mainly localized in a number of brain tissues such as the cortex, hippocampus and thalamus and has been noted for its contribution in generating neuronal excitation and seizures. Recently, it has been also proposed that hCA VII may be involved in the control of neuropathic pain, thus its inhibition may offer a new approach in designing pain killers useful for combating neuropathic pain. We report here the X-ray crystallographic structure of a mutated form of human CA VII in complex with acetazolamide, a classical sulfonamide inhibitor. These crystallographic studies provide important implications for the rational drug design of selective CA inhibitors with clinical applications.

PubMed: 20688517
DOI: 10.1016/j.bmcl.2010.07.051

実験手法

X-RAY DIFFRACTION (2.05 Å)