3MIS

I-MsoI re-designed for altered DNA cleavage specificity (-8G)

Summary for 3MIS

Related2ko2 2mip
DescriptorMso-8G, DNA (5'-D(*GP*CP*AP*GP*GP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*CP*TP*CP*CP*G)-3'), DNA (5'-D(*CP*GP*GP*AP*GP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*CP*CP*TP*GP*C)-3'), ... (5 entities in total)
Functional Keywordsprotein-dna complex, homing nuclease, rosetta design, de novo protein-dna complex, de novo protein/dna
Biological sourcesynthetic construct (artificial gene)
Total number of polymer chains4
Total molecular weight52124.58
Authors
Taylor, G.K.,Stoddard, B.L. (deposition date: 2010-04-12, release date: 2010-05-19, Last modification date: 2011-07-13)
Primary citation
Ashworth, J.,Taylor, G.K.,Havranek, J.J.,Quadri, S.A.,Stoddard, B.L.,Baker, D.
Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.
Nucleic Acids Res., 38:5601-5608, 2010
PubMed: 20435674 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gkq283
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.289210.9%6.7%7.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution