3MI8

The structure of TL1A-DCR3 COMPLEX

3MI8 の概要

• エントリーDOI: 10.2210/pdb3mi8/pdb
• 関連するPDBエントリー: 2QE3 3K51 3MHD
• 分子名称: TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 15, SECRETED FORM, Tumor necrosis factor receptor superfamily member 6B (3 entities in total)
• 機能のキーワード: dcr3, tl1a, tnf, tnfr, decoy receptor, immunity, disulfide bond, glycoprotein, secreted, receptor, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type II membrane protein (Probable). Tumor necrosis factor ligand superfamily member 15, secreted form: Secreted: O95150; Secreted: O95407
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40182.17

構造登録者

Zhan, C.,Patskovsky, Y.,Yan, Q.,Li, Z.,Ramagopal, U.A.,Nathenson, S.G.,Almo, S.C. (登録日: 2010-04-09, 公開日: 2011-02-23, 最終更新日: 2024-11-27)

主引用文献

Zhan, C.,Patskovsky, Y.,Yan, Q.,Li, Z.,Ramagopal, U.,Cheng, H.,Brenowitz, M.,Hui, X.,Nathenson, S.G.,Almo, S.C.
Decoy Strategies: The Structure of TL1A:DcR3 Complex.
Structure, 19:162-171, 2011

PubMed Abstract: Decoy Receptor 3 (DcR3), a secreted member of the Tumor Necrosis Factor (TNF) receptor superfamily, neutralizes three different TNF ligands: FasL, LIGHT, and TL1A. Each of these ligands engages unique signaling receptors which direct distinct and critical immune responses. We report the crystal structures of the unliganded DcR3 ectodomain and its complex with TL1A, as well as complementary mutagenesis and biochemical studies. These analyses demonstrate that DcR3 interacts with invariant backbone and side-chain atoms in the membrane-proximal half of TL1A which supports recognition of its three distinct TNF ligands. Additional features serve as antideterminants that preclude interaction with other members of the TNF superfamily. This mode of interaction is unique among characterized TNF:TNFR family members and provides a mechanistic basis for the broadened specificity required to support the decoy function of DcR3, as well as for the rational manipulation of specificity and affinity of DcR3 and its ligands.

PubMed: 21300286
DOI: 10.1016/j.str.2010.12.004

実験手法

X-RAY DIFFRACTION (2.951 Å)