3MEX

Crystal structure of MexR in oxidized state

Summary for 3MEX

• Entry DOI: 10.2210/pdb3mex/pdb
• Related: 1LNW 3ECH
• Descriptor: Multidrug resistance operon repressor (2 entities in total)
• Functional Keywords: antibiotics resistance regulation, oxidative stress, thiol modification, dna-binding, transcription, transcription regulation
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 2
• Total formula weight: 32807.70

Authors

Chen, H.,Yi, C.,Zhang, J.,Zhang, W.,Yang, C.-G.,He, C. (deposition date: 2010-04-01, release date: 2010-07-28, Last modification date: 2024-10-30)

Primary citation

Chen, H.,Yi, C.,Zhang, J.,Zhang, W.,Ge, Z.,Yang, C.-G.,He, C.
Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR
Embo Rep., 11:685-690, 2010

PubMed Abstract: MexR functions as the primary regulator of the mexAB-oprM multidrug efflux expression in Pseudomonas aeruginosa. It has been shown that MexR senses oxidative stress by interprotomer disulphide bond formation between redox-active cysteines. This oxidation induces MexR to dissociate from the promoter DNA, thus activating the transcriptional expression of efflux pump genes. In this study, we present the crystal structure of MexR in its oxidized form at a resolution of 2.1 A. This crystal structure reveals the mechanism by which oxidative signal allosterically derepresses the MexR-controlled transcription activation.

PubMed: 20616806
DOI: 10.1038/embor.2010.96

Experimental method

X-RAY DIFFRACTION (2.1 Å)