3MDC

DNA polymerase lambda in complex with dFdCTP

3MDC の概要

• エントリーDOI: 10.2210/pdb3mdc/pdb
• 関連するPDBエントリー: 3MDA
• 分子名称: DNA polymerase lambda, DNA (5'-D(*CP*GP*GP*CP*GP*GP*TP*AP*CP*TP*G)-3'), DNA (5'-D(*CP*AP*GP*TP*AP*C)-3'), ... (8 entities in total)
• 機能のキーワード: protein-dna complex, lyase, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus : Q9UGP5
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 43424.52

構造登録者

Garcia-Diaz, M.,Murray, M.,Kunkel, T.,Chou, K.M. (登録日: 2010-03-30, 公開日: 2010-04-28, 最終更新日: 2024-02-21)

主引用文献

Garcia-Diaz, M.,Murray, M.S.,Kunkel, T.A.,Chou, K.M.
Interaction between DNA Polymerase lambda and anticancer nucleoside analogs.
J.Biol.Chem., 285:16874-16879, 2010

PubMed Abstract: The anticancer activity of cytarabine (AraC) and gemcitabine (dFdC) is thought to result from chain termination after incorporation into DNA. To investigate their incorporation into DNA at atomic level resolution, we present crystal structures of human DNA polymerase lambda (Pol lambda) bound to gapped DNA and containing either AraC or dFdC paired opposite template dG. These structures reveal that AraC and dFdC can bind within the nascent base pair binding pocket of Pol lambda. Although the conformation of the ribose of AraCTP is similar to that of normal dCTP, the conformation of dFdCTP is significantly different. Consistent with these structures, Pol lambda efficiently incorporates AraCTP but not dFdCTP. The data are consistent with the possibility that Pol lambda could modulate the cytotoxic effect of AraC.

PubMed: 20348107
DOI: 10.1074/jbc.M109.094391

実験手法

X-RAY DIFFRACTION (1.999 Å)