3M3J

A new crystal form of Lys48-linked diubiquitin

3M3J の概要

• エントリーDOI: 10.2210/pdb3m3j/pdb
• 分子名称: Ubiquitin, 1,2-ETHANEDIOL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: beta grasp, ubiquitin, isopeptide bond, nucleus, protein degradation, proteasome, signaling protein, lys48-linked
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 51811.24

構造登録者

Trempe, J.F.,Brown, N.R.,Noble, M.E.M.,Endicott, J.A. (登録日: 2010-03-09, 公開日: 2010-03-23, 最終更新日: 2024-11-20)

主引用文献

Trempe, J.F.,Brown, N.R.,Noble, M.E.,Endicott, J.A.
A new crystal form of Lys48-linked diubiquitin.
Acta Crystallogr.,Sect.F, 66:994-998, 2010

PubMed Abstract: Lys48-linked polyubiquitin chains are recognized by the proteasome as a tag for the degradation of the attached substrates. Here, a new crystal form of Lys48-linked diubiquitin (Ub2) was obtained and the crystal structure was refined to 1.6 A resolution. The structure reveals an ordered isopeptide bond in a trans configuration. All three molecules in the asymmetric unit were in the same closed conformation, in which the hydrophobic patches of both the distal and the proximal moieties interact with each other. Despite the different crystallization conditions and different crystal packing, the new crystal structure of Ub2 is similar to the previously published structure of diubiquitin, but differences are observed in the conformation of the flexible isopeptide linkage.

PubMed: 20823512
DOI: 10.1107/S1744309110027600

実験手法

X-RAY DIFFRACTION (1.6 Å)