3LNM

F233W mutant of the Kv2.1 paddle-Kv1.2 chimera channel

3LNM の概要

• エントリーDOI: 10.2210/pdb3lnm/pdb
• 関連するPDBエントリー: 2R9R
• 分子名称: Voltage-gated potassium channel subunit beta-2, F233W mutant of the Kv2.1 paddle-Kv1.2 chimera, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (6 entities in total)
• 機能のキーワード: voltage-gated potassium channel-beta subunit complex, acetylation, cytoplasm, ion transport, ionic channel, nadp, phosphoprotein, potassium, potassium transport, transport, voltage-gated channel, glycoprotein, lipoprotein, membrane, palmitate, potassium channel, transmembrane, membrane protein, transport protein
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats); 詳細
• 細胞内の位置: Cytoplasm : P62483; Cell membrane ; Multi-pass membrane protein : P63142
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 204042.48

構造登録者

Tao, X.,Lee, A.,Limapichat, W.,Dougherty, D.A.,MacKinnon, R. (登録日: 2010-02-02, 公開日: 2010-04-14, 最終更新日: 2023-09-06)

主引用文献

Tao, X.,Lee, A.,Limapichat, W.,Dougherty, D.A.,MacKinnon, R.
A gating charge transfer center in voltage sensors.
Science, 328:67-73, 2010

PubMed Abstract: Voltage sensors regulate the conformations of voltage-dependent ion channels and enzymes. Their nearly switchlike response as a function of membrane voltage comes from the movement of positively charged amino acids, arginine or lysine, across the membrane field. We used mutations with natural and unnatural amino acids, electrophysiological recordings, and x-ray crystallography to identify a charge transfer center in voltage sensors that facilitates this movement. This center consists of a rigid cyclic "cap" and two negatively charged amino acids to interact with a positive charge. Specific mutations induce a preference for lysine relative to arginine. By placing lysine at specific locations, the voltage sensor can be stabilized in different conformations, which enables a dissection of voltage sensor movements and their relation to ion channel opening.

PubMed: 20360102
DOI: 10.1126/science.1185954

実験手法

X-RAY DIFFRACTION (2.9 Å)