3LK8

Crystal structure of the catalytic domain of human MMP12 complexed with the inhibitor paramethoxy-sulfonyl-glycine hydroxamate

Summary for 3LK8

• Entry DOI: 10.2210/pdb3lk8/pdb
• Related: 3F15 3F16 3F17 3F18 3F19 3F1A
• Descriptor: Macrophage metalloelastase, ZINC ION, CALCIUM ION, ... (5 entities in total)
• Functional Keywords: matrix metalloproteinase, metallo elastase, elastase inhibitor, glycoprotein, hydrolase, metal-binding, metalloprotease, protease
• Biological source: Homo sapiens (human)
• Cellular location: Secreted, extracellular space, extracellular matrix : P39900
• Total number of polymer chains: 1
• Total formula weight: 17995.79

Authors

Calderone, V. (deposition date: 2010-01-27, release date: 2010-10-06, Last modification date: 2023-09-06)

Primary citation

Bertini, I.,Calderone, V.,Fragai, M.,Giachetti, A.,Loconte, M.,Luchinat, C.,Maletta, M.,Nativi, C.,Yeo, K.J.
Exploring the subtleties of drug-receptor interactions: the case of matrix metalloproteinases
J.Am.Chem.Soc., 129:2466-2475, 2007

PubMed Abstract: By solving high-resolution crystal structures of a large number (14 in this case) of adducts of matrix metalloproteinase 12 (MMP12) with strong, nanomolar, inhibitors all derived from a single ligand scaffold, it is shown that the energetics of the ligand-protein interactions can be accounted for directly from the structures to a level of detail that allows us to rationalize for the differential binding affinity between pairs of closely related ligands. In each case, variations in binding affinities can be traced back to slight improvements or worsening of specific interactions with the protein of one or more ligand atoms. Isothermal calorimetry measurements show that the binding of this class of MMP inhibitors is largely enthalpy driven, but a favorable entropic contribution is always present. The binding enthalpy of acetohydroxamic acid (AHA), the prototype zinc-binding group in MMP drug discovery, has been also accurately measured. In principle, this research permits the planning of either improved inhibitors, or inhibitors with improved selectivity for one or another MMP. The present analysis is applicable to any drug target for which structural information on adducts with a series of homologous ligands can be obtained, while structural information obtained from in silico docking is probably not accurate enough for this type of study.

PubMed: 17269766

Experimental method

X-RAY DIFFRACTION (1.8 Å)