3LG8
Crystal structure of the C-terminal part of subunit E (E101-206) from Methanocaldococcus jannaschii of A1AO ATP synthase
Summary for 3LG8
| Entry DOI | 10.2210/pdb3lg8/pdb |
| Related | 2DM9 2DMA |
| Descriptor | A-type ATP synthase subunit E (1 entity in total) |
| Functional Keywords | archaea, peripheral stalk, hydrolase, structural protein, transport protein |
| Biological source | Methanocaldococcus jannaschii (Methanococcus jannaschii) |
| Total number of polymer chains | 2 |
| Total formula weight | 23709.37 |
| Authors | Balakrishna, A.M.,Manimekalai, M.S.S.,Hunke, C.,Gayen, S.,Jeyakanthan, J.,Gruber, G. (deposition date: 2010-01-19, release date: 2010-07-07, Last modification date: 2023-11-01) |
| Primary citation | Balakrishna, A.M.,Manimekalai, M.S.S.,Hunke, C.,Gayen, S.,Rossle, M.,Jeyakanthan, J.,Gruber, G. Crystal and solution structure of the C-terminal part of the Methanocaldococcus jannaschii A1AO ATP synthase subunit E revealed by X-ray diffraction and small-angle X-ray scattering J.Bioenerg.Biomembr., 42:311-320, 2010 Cited by PubMed Abstract: The structure of the C-terminus of subunit E (E(101-206)) of Methanocaldococcus jannaschii A-ATP synthase was determined at 4.1 A. E(101-206) consist of a N-terminal globular domain with three alpha-helices and four antiparallel beta-strands and an alpha-helix at the very C-terminus. Comparison of M. jannaschii E(101-206) with the C-terminus E(81-198) subunit E from Pyrococcus horikoshii OT3 revealed that the kink in the C-terminal alpha-helix of E(81-198), involved in dimer formation, is absent in M. jannaschii E(101-206). Whereas a major dimeric surface interface is present between the P. horikoshii E(81-198) molecules in the asymmetric unit, no such interaction could be found in the M. jannaschii E(101-206) molecules. To verify the oligomeric behaviour, the low resolution structure of the recombinant E(85-206) from M. jannaschii was determined using small angle X-ray scattering. Rigid body modeling of two copies of one of the monomer established a fit with a tail to tail arrangement. PubMed: 20571891DOI: 10.1007/s10863-010-9298-3 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (4.1 Å) |
Structure validation
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