3L1P

POU protein:DNA complex

Summary for 3L1P

• Entry DOI: 10.2210/pdb3l1p/pdb
• Descriptor: POU domain, class 5, transcription factor 1, DNA (5'-D(*TP*CP*CP*AP*CP*AP*TP*TP*TP*GP*AP*AP*AP*GP*GP*CP*AP*AP*AP*TP*GP*GP*A)-3'), DNA (5'-D(*AP*TP*CP*CP*AP*TP*TP*TP*GP*CP*CP*TP*TP*TP*CP*AP*AP*AP*TP*GP*TP*GP*G)-3') (3 entities in total)
• Functional Keywords: pou, transcription factor dna complex, pore, stem cells, embryonic, transcription-dna complex, transcription/dna
• Biological source: Mus musculus (mouse); More
• Cellular location: Cytoplasm : P20263
• Total number of polymer chains: 4
• Total formula weight: 50044.92

Authors

Vahokoski, J.,Groves, M.R.,Pogenberg, V.,Wilmanns, M. (deposition date: 2009-12-14, release date: 2010-12-15, Last modification date: 2023-11-01)

Primary citation

Esch, D.,Vahokoski, J.,Groves, M.R.,Pogenberg, V.,Cojocaru, V.,Vom Bruch, H.,Han, D.,Drexler, H.C.A.,Arauzo-Bravo, M.J.,Ng, C.K.L.,Jauch, R.,Wilmanns, M.,Scholer, H.R.
A unique Oct4 interface is crucial for reprogramming to pluripotency
Nat.Cell Biol., 15:295-301, 2013

PubMed Abstract: Terminally differentiated cells can be reprogrammed to pluripotency by the forced expression of Oct4, Sox2, Klf4 and c-Myc. However, it remains unknown how this leads to the multitude of epigenetic changes observed during the reprogramming process. Interestingly, Oct4 is the only factor that cannot be replaced by other members of the same family to induce pluripotency. To understand the unique role of Oct4 in reprogramming, we determined the structure of its POU domain bound to DNA. We show that the linker between the two DNA-binding domains is structured as an α-helix and exposed to the protein's surface, in contrast to the unstructured linker of Oct1. Point mutations in this α-helix alter or abolish the reprogramming activity of Oct4, but do not affect its other fundamental properties. On the basis of mass spectrometry studies of the interactome of wild-type and mutant Oct4, we propose that the linker functions as a protein-protein interaction interface and plays a crucial role during reprogramming by recruiting key epigenetic players to Oct4 target genes. Thus, we provide molecular insights to explain how Oct4 contributes to the reprogramming process.

PubMed: 23376973
DOI: 10.1038/ncb2680

Experimental method

X-RAY DIFFRACTION (2.8 Å)