3L1P

POU protein:DNA complex

3L1P の概要

• エントリーDOI: 10.2210/pdb3l1p/pdb
• 分子名称: POU domain, class 5, transcription factor 1, DNA (5'-D(*TP*CP*CP*AP*CP*AP*TP*TP*TP*GP*AP*AP*AP*GP*GP*CP*AP*AP*AP*TP*GP*GP*A)-3'), DNA (5'-D(*AP*TP*CP*CP*AP*TP*TP*TP*GP*CP*CP*TP*TP*TP*CP*AP*AP*AP*TP*GP*TP*GP*G)-3') (3 entities in total)
• 機能のキーワード: pou, transcription factor dna complex, pore, stem cells, embryonic, transcription-dna complex, transcription/dna
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Cytoplasm : P20263
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 50044.92

構造登録者

Vahokoski, J.,Groves, M.R.,Pogenberg, V.,Wilmanns, M. (登録日: 2009-12-14, 公開日: 2010-12-15, 最終更新日: 2023-11-01)

主引用文献

Esch, D.,Vahokoski, J.,Groves, M.R.,Pogenberg, V.,Cojocaru, V.,Vom Bruch, H.,Han, D.,Drexler, H.C.A.,Arauzo-Bravo, M.J.,Ng, C.K.L.,Jauch, R.,Wilmanns, M.,Scholer, H.R.
A unique Oct4 interface is crucial for reprogramming to pluripotency
Nat.Cell Biol., 15:295-301, 2013

PubMed Abstract: Terminally differentiated cells can be reprogrammed to pluripotency by the forced expression of Oct4, Sox2, Klf4 and c-Myc. However, it remains unknown how this leads to the multitude of epigenetic changes observed during the reprogramming process. Interestingly, Oct4 is the only factor that cannot be replaced by other members of the same family to induce pluripotency. To understand the unique role of Oct4 in reprogramming, we determined the structure of its POU domain bound to DNA. We show that the linker between the two DNA-binding domains is structured as an α-helix and exposed to the protein's surface, in contrast to the unstructured linker of Oct1. Point mutations in this α-helix alter or abolish the reprogramming activity of Oct4, but do not affect its other fundamental properties. On the basis of mass spectrometry studies of the interactome of wild-type and mutant Oct4, we propose that the linker functions as a protein-protein interaction interface and plays a crucial role during reprogramming by recruiting key epigenetic players to Oct4 target genes. Thus, we provide molecular insights to explain how Oct4 contributes to the reprogramming process.

PubMed: 23376973
DOI: 10.1038/ncb2680

実験手法

X-RAY DIFFRACTION (2.8 Å)