3KSA

Detailed structural insight into the DNA cleavage complex of type IIA topoisomerases (cleaved form)

3KSA の概要

• エントリーDOI: 10.2210/pdb3ksa/pdb
• 関連するPDBエントリー: 2NOV 3FOE 3FOF 3K9F 3KSB
• 分子名称: DNA topoisomerase 4 subunit A, DNA topoisomerase 4 subunit B, 5'-D(*AP*CP*CP*AP*AP*GP*GP*T*CP*AP*TP*GP*AP*AP*T)-3', ... (8 entities in total)
• 機能のキーワード: topoisomerase, protein-dna cleavage complex, streptococcus pneumoniae, dna-binding, nucleotide-binding, quinolone, cleaved form, isomerase-dna complex, isomerase/dna
• 由来する生物種: Streptococcus pneumoniae; 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein (By similarity): P72525
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 194616.47

構造登録者

Laponogov, I.,Pan, X.-S.,Veselkov, D.A.,McAuley, K.E.,Fisher, L.M.,Sanderson, M.R. (登録日: 2009-11-21, 公開日: 2010-05-05, 最終更新日: 2024-10-30)

主引用文献

Laponogov, I.,Pan, X.-S.,Veselkov, D.A.,McAuley, K.E.,Fisher, L.M.,Sanderson, M.R.
Structural Basis of Gate-DNA Breakage and Resealing by Type II Topoisomerases
Plos One, 5:e11338-e11338, 2010

PubMed Abstract: Type II DNA topoisomerases are ubiquitous enzymes with essential functions in DNA replication, recombination and transcription. They change DNA topology by forming a transient covalent cleavage complex with a gate-DNA duplex that allows transport of a second duplex though the gate. Despite its biological importance and targeting by anticancer and antibacterial drugs, cleavage complex formation and reversal is not understood for any type II enzyme. To address the mechanism, we have used X-ray crystallography to study sequential states in the formation and reversal of a DNA cleavage complex by topoisomerase IV from Streptococcus pneumoniae, the bacterial type II enzyme involved in chromosome segregation. A high resolution structure of the complex captured by a novel antibacterial dione reveals two drug molecules intercalated at a cleaved B-form DNA gate and anchored by drug-specific protein contacts. Dione release generated drug-free cleaved and resealed DNA complexes in which the DNA gate instead adopts an unusual A/B-form helical conformation with a Mg(2+) ion repositioned to coordinate each scissile phosphodiester group and promote reversible cleavage by active-site tyrosines. These structures, the first for putative reaction intermediates of a type II topoisomerase, suggest how a type II enzyme reseals DNA during its normal reaction cycle and illuminate aspects of drug arrest important for the development of new topoisomerase-targeting therapeutics.

PubMed: 20596531
DOI: 10.1371/journal.pone.0011338

実験手法

X-RAY DIFFRACTION (3.3 Å)