3KSA
Detailed structural insight into the DNA cleavage complex of type IIA topoisomerases (cleaved form)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA topoisomerase 4 subunit A | polymer | 496 | 56455.4 | 2 | UniProt (P72525) Pfam (PF00521) In PDB | Streptococcus pneumoniae | ParC55, Topoisomerase IV subunit A |
| 2 | C, D | DNA topoisomerase 4 subunit B | polymer | 268 | 30415.7 | 2 | UniProt (Q59961) Pfam (PF01751) Pfam (PF00986) In PDB | Streptococcus pneumoniae | ParE30, Topoisomerase IV subunit B |
| 3 | E | 5'-D(*AP*CP*CP*AP*AP*GP*GP*T*CP*AP*TP*GP*AP*AP*T)-3' | polymer | 15 | 4602.0 | 1 | |||
| 4 | F | 5'-D(P*AP*GP*TP*CP*AP*TP*TP*CP*AP*TP*GP*AP*CP*CP*TP*TP*GP*GP*T)-3' | polymer | 19 | 5810.8 | 1 | |||
| 5 | G | 5'-D(*CP*TP*GP*TP*TP*TP*TP*A*CP*GP*TP*GP*CP*AP*T)-3' | polymer | 15 | 4566.0 | 1 | |||
| 6 | H | 5'-D(P*GP*AP*CP*TP*AP*TP*GP*CP*AP*CP*GP*TP*AP*AP*AP*AP*CP*AP*G)-3' | polymer | 19 | 5846.8 | 1 | |||
| 7 | C, D | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 8 | water | water | 18.0 | 7 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 488 (UniProt: P72525)
C, D: 404 - 647 (UniProt: Q59961)
| PDB | External Database | Details |
|---|---|---|
| Leu 489 | - | expression tag |
| Glu 490 | - | expression tag |
| His 491 | - | expression tag |
| His 492 | - | expression tag |
| His 493 | - | expression tag |
| His 494 | - | expression tag |
| His 495 | - | expression tag |
| His 496 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 380 | - | initiating methionine |
| Gly 381 | - | expression tag |
| His 382 | - | expression tag |
| His 383 | - | expression tag |
| His 384 | - | expression tag |
| His 385 | - | expression tag |
| His 386 | - | expression tag |
| His 387 | - | expression tag |
| His 388 | - | expression tag |
| His 389 | - | expression tag |
| His 390 | - | expression tag |
| His 391 | - | expression tag |
| Ser 392 | - | expression tag |
| Ser 393 | - | expression tag |
| Gly 394 | - | expression tag |
| His 395 | - | expression tag |
| Ile 396 | - | expression tag |
| Asp 397 | - | expression tag |
| Asp 398 | - | expression tag |
| Asp 399 | - | expression tag |
| Asp 400 | - | expression tag |
| Lys 401 | - | expression tag |
| His 402 | - | expression tag |
| Met 403 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 194567.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 194616.5 |
