3KRB

Structure of Aldose Reductase from Giardia Lamblia at 1.75A Resolution

Summary for 3KRB

• Entry DOI: 10.2210/pdb3krb/pdb
• Descriptor: Aldose reductase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, UNKNOWN ATOM OR ION, ... (5 entities in total)
• Functional Keywords: ssgcid, sbri, emerald biostructures, university of washington, nih, niaid, aldose reductase, giardia lamblia, oxidoreductase, structural genomics, seattle structural genomics center for infectious disease
• Biological source: Giardia lamblia
• Total number of polymer chains: 2
• Total formula weight: 77209.27

Authors

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2009-11-18, release date: 2009-12-01, Last modification date: 2023-09-06)

Primary citation

Ferrell, M.,Abendroth, J.,Zhang, Y.,Sankaran, B.,Edwards, T.E.,Staker, B.L.,Van Voorhis, W.C.,Stewart, L.J.,Myler, P.J.
Structure of aldose reductase from Giardia lamblia.
Acta Crystallogr.,Sect.F, 67:1113-1117, 2011

PubMed Abstract: Giardia lamblia is an anaerobic aerotolerant eukaryotic parasite of the intestines. It is believed to have diverged early from eukarya during evolution and is thus lacking in many of the typical eukaryotic organelles and biochemical pathways. Most conspicuously, mitochondria and the associated machinery of oxidative phosphorylation are absent; instead, energy is derived from substrate-level phosphorylation. Here, the 1.75 Å resolution crystal structure of G. lamblia aldose reductase heterologously expressed in Escherichia coli is reported. As in other oxidoreductases, G. lamblia aldose reductase adopts a TIM-barrel conformation with the NADP(+)-binding site located within the eight β-strands of the interior.

PubMed: 21904059
DOI: 10.1107/S1744309111030879

Experimental method

X-RAY DIFFRACTION (1.75 Å)