3KQ4

Structure of Intrinsic Factor-Cobalamin bound to its receptor Cubilin

3KQ4 の概要

• エントリーDOI: 10.2210/pdb3kq4/pdb
• 関連するPDBエントリー: 2PMW
• 分子名称: Gastric intrinsic factor, Cubilin, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: protein-protein complex, cobalt, cobalt transport, disease mutation, disulfide bond, glycoprotein, secreted, transport, cholesterol metabolism, cobalamin, egf-like domain, endocytosis, endosome, lipid metabolism, lysosome, membrane, protein transport, receptor, steroid metabolism, transport protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 299190.07

構造登録者

Andersen, C.B.F.,Madsen, M.,Moestrup, S.K.,Andersen, G.R. (登録日: 2009-11-17, 公開日: 2010-03-09, 最終更新日: 2024-10-30)

主引用文献

Andersen, C.B.,Madsen, M.,Storm, T.,Moestrup, S.K.,Andersen, G.R.
Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes.
Nature, 464:445-448, 2010

PubMed Abstract: Cobalamin (Cbl, vitamin B(12)) is a bacterial organic compound and an essential coenzyme in mammals, which take it up from the diet. This occurs by the combined action of the gastric intrinsic factor (IF) and the ileal endocytic cubam receptor formed by the 460-kilodalton (kDa) protein cubilin and the 45-kDa transmembrane protein amnionless. Loss of function of any of these proteins ultimately leads to Cbl deficiency in man. Here we present the crystal structure of the complex between IF-Cbl and the cubilin IF-Cbl-binding-region (CUB(5-8)) determined at 3.3 A resolution. The structure provides insight into how several CUB (for 'complement C1r/C1s, Uegf, Bmp1') domains collectively function as modular ligand-binding regions, and how two distant CUB domains embrace the Cbl molecule by binding the two IF domains in a Ca(2+)-dependent manner. This dual-point model provides a probable explanation of how Cbl indirectly induces ligand-receptor coupling. Finally, the comparison of Ca(2+)-binding CUB domains and the low-density lipoprotein (LDL) receptor-type A modules suggests that the electrostatic pairing of a basic ligand arginine/lysine residue with Ca(2+)-coordinating acidic aspartates/glutamates is a common theme of Ca(2+)-dependent ligand-receptor interactions.

PubMed: 20237569
DOI: 10.1038/nature08874

実験手法

X-RAY DIFFRACTION (3.3 Å)