3KO2

I-MsoI re-designed for altered DNA cleavage specificity (-7C)

Summary for 3KO2

• Entry DOI: 10.2210/pdb3ko2/pdb
• Descriptor: Site-specific DNA endonuclease I-MsoI, 5'-D(*GP*CP*AP*GP*AP*CP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3', 5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*GP*TP*CP*TP*GP*C)-3', ... (5 entities in total)
• Functional Keywords: redesign, protein-dna complex, chloroplast, endonuclease, hydrolase-dna complex, hydrolase/dna
• Biological source: Monomastix sp.; More
• Total number of polymer chains: 8
• Total formula weight: 108131.54

Authors

Taylor, G.K.,Stoddard, B.L. (deposition date: 2009-11-13, release date: 2010-05-19, Last modification date: 2023-09-06)

• Primary citation: Ashworth, J.,Taylor, G.K.,Havranek, J.J.,Quadri, S.A.,Stoddard, B.L.,Baker, D.; Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.; Nucleic Acids Res., 38:5601-5608, 2010; PubMed: 20435674; DOI: 10.1093/nar/gkq283

Experimental method

X-RAY DIFFRACTION (2.9 Å)