3KLO

Vibrio cholerae VpsT bound to c-di-GMP

3KLO の概要

• エントリーDOI: 10.2210/pdb3klo/pdb
• 関連するPDBエントリー: 3KLN
• 分子名称: Transcriptional regulator VpsT, 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one), D(-)-TARTARIC ACID, ... (4 entities in total)
• 機能のキーワード: rec domain, hth domain, dna-binding, transcription, transcription regulation
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 106933.83

構造登録者

Krasteva, P.V.,Navarro, V.A.S.,Sondermann, H. (登録日: 2009-11-08, 公開日: 2010-02-09, 最終更新日: 2023-09-06)

主引用文献

Krasteva, P.V.,Fong, J.C.,Shikuma, N.J.,Beyhan, S.,Navarro, M.V.,Yildiz, F.H.,Sondermann, H.
Vibrio cholerae VpsT Regulates Matrix Production and Motility by Directly Sensing Cyclic di-GMP.
Science, 327:866-868, 2010

PubMed Abstract: Microorganisms can switch from a planktonic, free-swimming life-style to a sessile, colonial state, called a biofilm, which confers resistance to environmental stress. Conversion between the motile and biofilm life-styles has been attributed to increased levels of the prokaryotic second messenger cyclic di-guanosine monophosphate (c-di-GMP), yet the signaling mechanisms mediating such a global switch are poorly understood. Here we show that the transcriptional regulator VpsT from Vibrio cholerae directly senses c-di-GMP to inversely control extracellular matrix production and motility, which identifies VpsT as a master regulator for biofilm formation. Rather than being regulated by phosphorylation, VpsT undergoes a change in oligomerization on c-di-GMP binding.

PubMed: 20150502
DOI: 10.1126/science.1181185

実験手法

X-RAY DIFFRACTION (2.802 Å)