3KJF

Caspase 3 Bound to a covalent inhibitor

3KJF の概要

• エントリーDOI: 10.2210/pdb3kjf/pdb
• 関連するPDBエントリー: 3KJN 3KJQ
• 分子名称: Caspase-3, (3S)-3-({[(5S,10aS)-2-{(2S)-4-carboxy-2-[(phenylacetyl)amino]butyl}-1,3-dioxo-2,3,5,7,8,9,10,10a-octahydro-1H-[1,2,4]triazolo[1,2-a]cinnolin-5-yl]carbonyl}amino)-4-oxopentanoic acid, ... (4 entities in total)
• 機能のキーワード: caspase 3, apoptosis, kinetics, peptidomimetic inhibitor, urazole, phosphoprotein, protease, s-nitrosylation, thiol protease, zymogen, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: P42574 P42574
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30101.12

構造登録者

Kamtekar, S.,Watt, W.,Finzel, B.C.,Harris, M.S.,Blinn, J.,Wang, Z.,Tomasselli, A.G. (登録日: 2009-11-03, 公開日: 2010-08-11, 最終更新日: 2024-11-20)

主引用文献

Wang, Z.,Watt, W.,Brooks, N.A.,Harris, M.S.,Urban, J.,Boatman, D.,McMillan, M.,Kahn, M.,Heinrikson, R.L.,Finzel, B.C.,Wittwer, A.J.,Blinn, J.,Kamtekar, S.,Tomasselli, A.G.
Kinetic and structural characterization of caspase-3 and caspase-8 inhibition by a novel class of irreversible inhibitors.
Biochim.Biophys.Acta, 1804:1817-1831, 2010

PubMed Abstract: Because of their central role in programmed cell death, the caspases are attractive targets for developing new therapeutics against cancer and autoimmunity, myocardial infarction and ischemic damage, and neurodegenerative diseases. We chose to target caspase-3, an executioner caspase, and caspase-8, an initiator caspase, based on the vast amount of information linking their functions to diseases. Through a structure-based drug design approach, a number of novel beta-strand peptidomimetic compounds were synthesized. Kinetic studies of caspase-3 and caspase-8 inhibition were carried out with these urazole ring-containing irreversible peptidomimetics and a known irreversible caspase inhibitor, Z-VAD-fmk. Using a stopped-flow fluorescence assay, we were able to determine individual kinetic parameters of caspase-3 and caspase-8 inhibition by these inhibitors. Z-VAD-fmk and the peptidomimetic inhibitors inhibit caspase-3 and caspase-8 via a three-step kinetic mechanism. Inhibition of both caspase-3 and caspase-8 by Z-VAD-fmk and of caspase-3 by the peptidomimetic inhibitors proceeds via two rapid equilibrium steps followed by a relatively fast inactivation step. However, caspase-8 inhibition by the peptidomimetics goes through a rapid equilibrium step, a slow-binding reversible step, and an extremely slow inactivation step. The crystal structures of inhibitor complexes of caspases-3 and -8 validate the design of the inhibitors by illustrating in detail how they mimic peptide substrates. One of the caspase-8 structures also shows binding at a secondary, allosteric site, providing a possible route to the development of noncovalent small molecule modulators of caspase activity.

PubMed: 20580860
DOI: 10.1016/j.bbapap.2010.05.007

実験手法

X-RAY DIFFRACTION (2 Å)